Document Detail

Effect of the alterations in the fusion protein of measles virus isolated from brains of patients with subacute sclerosing panencephalitis on syncytium formation.
MedLine Citation:
PMID:  17825451     Owner:  NLM     Status:  MEDLINE    
Measles virus (MV) is the causative agent of subacute sclerosing panencephalitis (SSPE) and viruses isolated from brains of the patients contain numerous mutations. We have previously demonstrated that the hemagglutinin (H) protein of MV SSPE strains can interact with the signaling lymphocyte activation molecule (SLAM) and an unidentified molecule on Vero cells, but not with CD46, as a receptor. The mechanism by which MV SSPE strains can induce cell-cell fusion in SLAM-negative Vero cells is not understood. We report here on the effect of mutations in the fusion (F) proteins of three MV SSPE strains on syncytium formation. The F proteins of the three SSPE strains were functional and co-expression with H protein from the MV wild-type or SSPE strains in this study induced formation of large syncytia in Vero cells as well as in cell lines expressing SLAM or CD46. Expression of chimeric F proteins of SSPE strains showed that amino acid substitutions in the F protein extracellular as well as cytoplasmic domain contributed to enhanced cell-cell fusion in Vero cells. These findings suggest a common molecular mechanism and a key role of the F protein for syncytium formation in cells expressing an unidentified third receptor for MV.
Minoru Ayata; Masashi Shingai; Xiaojun Ning; Misako Matsumoto; Tsukasa Seya; Sanae Otani; Toshiyuki Seto; Shinji Ohgimoto; Hisashi Ogura
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-09-07
Journal Detail:
Title:  Virus research     Volume:  130     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-11-12     Completed Date:  2008-01-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  260-8     Citation Subset:  IM    
Department of Virology, Osaka City University Medical School, Asahimachi, Abeno-ku, Osaka 545-8585, Japan.
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MeSH Terms
Amino Acid Substitution / genetics
Cercopithecus aethiops
Giant Cells / virology*
Measles virus / genetics*,  isolation & purification,  pathogenicity*
Mutant Proteins / genetics,  metabolism
Subacute Sclerosing Panencephalitis / virology*
Vero Cells
Viral Fusion Proteins / genetics*,  metabolism*
Reg. No./Substance:
0/Mutant Proteins; 0/Viral Fusion Proteins

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