Document Detail


Effect of alpha-tocopherol and ascorbic acid on bovine oocyte in vitro maturation.
MedLine Citation:
PMID:  15819954     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In vitro culture results in higher oxygen concentrations than in vivo environments, leading to an increased level of reactive oxygen species (ROS) that cause lipid peroxidation of cellular membranes. Alpha-tocopherol (active form of vitamin E) is an antioxidant that protects mammalian cells against lipid peroxidation, which is regenerated by ascorbic acid. The aim of this study was to determine the effect of the addition of alpha-tocopherol and/or ascorbic acid to the maturation medium on bovine oocyte in vitro maturation (IVM) and subsequently on in vitro fertilization (IVF) and embryo development. Cumulus-oocyte complexes (COCs) were matured in Medium 199 (control), and with the addition of alpha-tocopherol and/or ascorbic acid. The concentration of alpha-tocopherol in COCs was determined by high-performance liquid chromatography (HPLC). IVF and in vitro culture (IVC) were carried out in modified synthetic oviductal fluid (mSOF). The quantity of alpha-tocopherol naturally present in COCs diminished by half during IVM (p < 0.05), although in the presence of ascorbic acid it remained constant. A greater amount of alpha-tocopherol was detected in COCs matured in medium supplemented with this antioxidant (p < 0.05), but the addition of alpha-tocopherol plus ascorbic acid maintained higher levels of alpha-tocopherol (p < 0.05). Significant differences were not observed in the percentages of nuclear maturation and fertilization among different treatments. The presence of alpha-tocopherol or ascorbic acid in the maturation medium failed to modify the percentage of blastocysts obtained, unlike the addition of both antioxidants when a significant decrease was observed (p < 0.05). Absorbic acid maintained the antioxidant capacity of the alpha-tocopherol incorporated to COC membranes during IVM. The active form of vitamin E during maturation impaired the acquisition of oocyte developmental competence.
Authors:
G Dalvit; S P Llanes; A Descalzo; M Insani; M Beconi; P Cetica
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction in domestic animals = Zuchthygiene     Volume:  40     ISSN:  0936-6768     ISO Abbreviation:  Reprod. Domest. Anim.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-04-11     Completed Date:  2005-08-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9015668     Medline TA:  Reprod Domest Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  93-7     Citation Subset:  IM    
Affiliation:
Area of Biochemistry, School of Veterinary Sciences, University of Buenos Aires, Buenos Aires, Argentina. gadalvit@fvet.uba.ar
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MeSH Terms
Descriptor/Qualifier:
Animals
Antioxidants / pharmacology*
Ascorbic Acid / pharmacology*
Cattle / embryology,  physiology*
Cells, Cultured
Chromatography, High Pressure Liquid / veterinary
Coculture Techniques
Dose-Response Relationship, Drug
Drug Synergism
Female
Fertilization in Vitro / veterinary*
Lipid Peroxidation / drug effects
Oocytes / drug effects,  physiology*
Oxidative Stress / drug effects,  physiology
Reactive Oxygen Species / metabolism
alpha-Tocopherol / pharmacology*
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Reactive Oxygen Species; 50-81-7/Ascorbic Acid; 59-02-9/alpha-Tocopherol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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