| Effect of allyl isothiocyanate (AITC) in both nitrite- and nitrosamine-induced cell death, production of reactive oxygen species, and DNA damage by the single-cell gel electrophoresis (SCGE): does it have any protective effect on HepG2 cells? | |
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MedLine Citation:
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PMID: 20448263 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The current study was designed to investigate possible protective effect of allyl isothiocyanate (AITC) in nitrite- and nitrosamine-treated human hepatoma cells (HepG2) with the evaluation by cytotoxic effects and genotoxic effects determined by the single-cell gel electrophoresis (SCGE). Allyl isothiocyanate treatment enhanced cell viability and reduced intracellular reactive oxygen species (ROS) production in both nitrite- and nitrosamine-treated cells significantly. In SCGE, when compared to untreated control cells, all of the treated groups caused increases in the tail intensity (%) such as nitrite at 17%, N-nitrosodimethylamine (NDMA) at 279%, N-nitrosodiethylamine (NDEA) at 324%, and N-nitrosomorpholine (NMOR) at 288%. Allyl isothiocyanate reduced the tail intensity caused by nitrite 36%, by NDMA 36%, by NDEA 49%, and by NMOR 32%, respectively, when compared to each individual toxic compound-treated group. In conclusion, AITC protected HepG2 cells against cytotoxic and genotoxic effects caused by nitrite and the nitrosamines. |
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Authors:
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Pinar Erkekoğlu; Terken Baydar |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International journal of toxicology Volume: 29 ISSN: 1092-874X ISO Abbreviation: Int. J. Toxicol. Publication Date: 2010 May-Jun |
Date Detail:
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Created Date: 2010-05-07 Completed Date: 2010-08-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9708436 Medline TA: Int J Toxicol Country: United States |
Other Details:
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Languages: eng Pagination: 305-12 Citation Subset: IM |
Affiliation:
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Department of Toxicology, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey. erkekp@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants
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pharmacology* Cell Death / drug effects* Cell Survival / drug effects Comet Assay Cytotoxins / toxicity DNA Damage / drug effects* Hep G2 Cells Humans Isothiocyanates / pharmacology* Mutagens / toxicity Nitrites / toxicity* Nitrosamines / toxicity* Osmolar Concentration Oxidative Stress / drug effects Reactive Oxygen Species / metabolism* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Cytotoxins; 0/Isothiocyanates; 0/Mutagens; 0/Nitrites; 0/Nitrosamines; 0/Reactive Oxygen Species; 57-06-7/allyl isothiocyanate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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