Document Detail


Effect of allyl isothiocyanate (AITC) in both nitrite- and nitrosamine-induced cell death, production of reactive oxygen species, and DNA damage by the single-cell gel electrophoresis (SCGE): does it have any protective effect on HepG2 cells?
MedLine Citation:
PMID:  20448263     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The current study was designed to investigate possible protective effect of allyl isothiocyanate (AITC) in nitrite- and nitrosamine-treated human hepatoma cells (HepG2) with the evaluation by cytotoxic effects and genotoxic effects determined by the single-cell gel electrophoresis (SCGE). Allyl isothiocyanate treatment enhanced cell viability and reduced intracellular reactive oxygen species (ROS) production in both nitrite- and nitrosamine-treated cells significantly. In SCGE, when compared to untreated control cells, all of the treated groups caused increases in the tail intensity (%) such as nitrite at 17%, N-nitrosodimethylamine (NDMA) at 279%, N-nitrosodiethylamine (NDEA) at 324%, and N-nitrosomorpholine (NMOR) at 288%. Allyl isothiocyanate reduced the tail intensity caused by nitrite 36%, by NDMA 36%, by NDEA 49%, and by NMOR 32%, respectively, when compared to each individual toxic compound-treated group. In conclusion, AITC protected HepG2 cells against cytotoxic and genotoxic effects caused by nitrite and the nitrosamines.
Authors:
Pinar Erkekoğlu; Terken Baydar
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of toxicology     Volume:  29     ISSN:  1092-874X     ISO Abbreviation:  Int. J. Toxicol.     Publication Date:    2010 May-Jun
Date Detail:
Created Date:  2010-05-07     Completed Date:  2010-08-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9708436     Medline TA:  Int J Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  305-12     Citation Subset:  IM    
Affiliation:
Department of Toxicology, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey. erkekp@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Antioxidants / pharmacology*
Cell Death / drug effects*
Cell Survival / drug effects
Comet Assay
Cytotoxins / toxicity
DNA Damage / drug effects*
Hep G2 Cells
Humans
Isothiocyanates / pharmacology*
Mutagens / toxicity
Nitrites / toxicity*
Nitrosamines / toxicity*
Osmolar Concentration
Oxidative Stress / drug effects
Reactive Oxygen Species / metabolism*
Time Factors
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Cytotoxins; 0/Isothiocyanates; 0/Mutagens; 0/Nitrites; 0/Nitrosamines; 0/Reactive Oxygen Species; 57-06-7/allyl isothiocyanate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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