Document Detail

Effect of age on electrical field stimulation (EFS)-induced endothelium-dependent vasodilation in male and female rats.
MedLine Citation:
PMID:  11282086     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: The purpose of this study was to determine whether the effect of age on electrical field stimulation (EFS)-induced endothelium-dependent vasodilation was different in males and females. METHODS: Young (3 month) and old (25 month) Fisher 344 rats were studied: young females (YF, n=34), young males (YM, n=28), old females (OF, n=19), and old males (OM, n=24). Isolated mesenteric resistance arteries (endothelium-intact and denuded) were pressurized, and outer diameter was monitored. EFS-response curves (0.1-8 Hz) were performed in preconstricted arteries in the presence of guanethidine. EFS responses were expressed as percent relaxation from preconstricted diameter. Area under the curve (AUC) was calculated and comparisons were made using ANOVA and t-tests. RESULTS: Males became less responsive to EFS-induced vasodilation with age, while responses among females were unaffected (AUC: YM=344+/-23, OM=253+/-25, P=0.008; YF=397+/-21, OF=365+/-25, P=0.33). Endothelial denudation produced a significant decrease in EFS-induced dilation among all rat groups. The effect of denudation was greater in young animals compared to old. Incubation with the nitric oxide synthase inhibitor N(omega)-nitro-L-arginine (LNA) significantly decreased EFS responsiveness among all of the rat groups. CONCLUSIONS: EFS-induced vasodilation declines with age among males. In YF and YM endothelium-dependent EFS-induced vasodilation is mediated by the release of endothelium-derived NO. With age, endothelial function declines, which is likely due to a decrease in the production of endothelium-derived NO. A decline in endothelial-derived NO is likely responsible for the decrease in EFS-induced vasodilation with age among males.
J C Sullivan; C A Davison
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cardiovascular research     Volume:  50     ISSN:  0008-6363     ISO Abbreviation:  Cardiovasc. Res.     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-04-03     Completed Date:  2001-06-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0077427     Medline TA:  Cardiovasc Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  137-44     Citation Subset:  IM    
Center for Cardiovascular Sciences, Albany Medical College, Albany, NY, USA.
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MeSH Terms
Aging / physiology*
Capsaicin / pharmacology
Electric Stimulation
Endothelium, Vascular / drug effects,  physiology*
Enzyme Inhibitors / pharmacology
Nitric Oxide / physiology
Nitric Oxide Synthase / antagonists & inhibitors
Nitroarginine / pharmacology
Rats, Inbred F344
Tetrodotoxin / pharmacology
Vasodilation / drug effects,  physiology*
Grant Support
Reg. No./Substance:
0/Enzyme Inhibitors; 10102-43-9/Nitric Oxide; 2149-70-4/Nitroarginine; 404-86-4/Capsaicin; 4368-28-9/Tetrodotoxin; EC Oxide Synthase

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