Document Detail

Effect of administration of a recombinant adenovirus expressing the genes for IFN-gamma and interleukin-12 on acute murine toxoplasmosis.
MedLine Citation:
PMID:  11710988     Owner:  NLM     Status:  MEDLINE    
The effect of recombinant murine interferon-gamma (rMuIFN-gamma) produced from an adenovirus construct on Toxoplasma gondii in tissue culture and on the outcome of a T. gondii infection in mice was determined. Supernatants from AdCMVMuIFN-gamma-infected mouse lung epithelial (MuLE) cells were evaluated for the ability to produce biologically active IFN-gamma by measuring the capacity of the supernatants to activate peritoneal macrophages for killing of T. gondii. The bioactivity of IFN-gamma in supernatants increased with increasing multiplicity of infection (moi). Replication was inhibited 43%, 67%, and 70% by supernatants from MuLE cells infected with AdCMVMuIFN-gamma moi 5, 10, and 50, respectively, (p < 0.01 compared with controls). Bioactivity of IFN-gamma also increased as the length of time after infection increased. T. gondii replication was inhibited 28% and 36%, respectively, by AdCMVMuIFN-gamma-infected MuLE cell supernatants recovered at 24 and 48 h (p < 0.01 compared with control). In vivo administration of AdCMVMuIFN-gamma exhibited 33% mortality by day 9 in mice acutely infected with T. gondii compared with 100% mortality in control mice (p = 0.045). Administration of AdCMVIL-12 reduced mortality to 40% compared with control mice. However, this reduction was not significant (p = 0.08). Overall survival was extended 2 days with AdCMVMuINF-gamma administration and 5 days with AdCMVIL-12. AdCMVMuIFN-gamma in vitro inhibits T. gondii, and in vivo AdCMVMuIFN-gamma and AdCMVIL-12 lead to increased survival in mice.
D G Mack; H A Jaffe
Related Documents :
20442858 - Impaired innate immunity in tlr4(-/-) mice but preserved cd8+ t cell responses against ...
17211148 - Ip-10 is an early diagnostic marker for identification of late-onset bacterial infectio...
15153508 - Production of ifn-gamma by cd4 t cells is essential for resolving ehrlichia infection.
17220408 - The anti-trypanosoma cruzi activity of posaconazole in a murine model of acute chagas' ...
19826908 - Molecular interaction of α-synuclein with tubulin influences on the polymerization of m...
20174578 - Variable na(v)1.5 protein expression from the wild-type allele correlates with the pene...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research     Volume:  21     ISSN:  1079-9907     ISO Abbreviation:  J. Interferon Cytokine Res.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-11-16     Completed Date:  2002-02-01     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9507088     Medline TA:  J Interferon Cytokine Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  777-83     Citation Subset:  IM    
Department of Ophthalmology, University of Chicago, Chicago, IL 60637, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Acute Disease
Adenoviridae / genetics*
Cell Line
Cells, Cultured
Culture Media, Conditioned / pharmacology
DNA, Recombinant / administration & dosage
DNA, Viral / genetics
Gene Therapy*
Interferon-gamma / genetics*,  immunology,  metabolism
Interleukin-12 / genetics*,  metabolism
Macrophage Activation
Macrophages, Peritoneal / drug effects,  immunology
Respiratory Mucosa / immunology,  virology
Survival Analysis
Toxoplasma / drug effects,  growth & development
Toxoplasmosis, Animal / immunology,  therapy*
Transcription, Genetic
Reg. No./Substance:
0/Culture Media, Conditioned; 0/DNA, Recombinant; 0/DNA, Viral; 187348-17-0/Interleukin-12; 82115-62-6/Interferon-gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Complex bird clocks.
Next Document:  Ovine IFN-tau modulates the expression of MHC antigens on murine cerebrovascular endothelial cells a...