Document Detail

Effect of acute treadmill exercise on LFA-1 antigen expression in murine splenocytes.
MedLine Citation:
PMID:  8572588     Owner:  NLM     Status:  MEDLINE    
The LFA-1 intercellular adhesion molecule (ICAM), expressed on human and murine CD8+ and natural killer (NK) cells, participates in cytolytic interaction with target cells. This study was designed to determine whether acute exercise alters the activity of CD11a (LFA-1) on NK cells. Fourty male C3H/HeNHSD mice were sacrificed before (sedentary) or at various time points after acute treadmill exercise (30 m/min, 4 degrees slope, 30 minutes). Splenic natural killer cell activity (NKCA) was determined using a standard 51Cr release assay, using fresh splenocytes or splenocytes which had been incubated for 3 days with 100 U rIL-2. 10 micrograms/ml of mAb to LFA-1(anti-CD11a) or an equal volume of culture medium were added to the effector:target suspensions prior to 4.5 h incubation. There was a significant main effect of antibody treatment on cytolysis of tumour targets by fresh and IL-2 augmented splenocytes (p < 0.001); for both fresh and IL-2 augmented splenocytes, addition of anti-CD11a antibody reduced cytolysis of tumour target cells. Further, there was a significant main effect of exercise on fresh (p < 0.01) but not IL-2 augmented splenocytes cytolysis; this effect was due to differences in cytolytic activity between 30 and 120 minutes post exercise. Calculation of delta% in cytotoxicity showed that the capacity of mAb to CD11a to inhibit cytolysis was the lowest at 30 minutes after exercise (25%). The delta% in cytotoxicity was less pronounced in samples obtained from IL-2 augmented splenocytes, irrespective of timing of exercise. These results suggest that acute exercise renders mouse splenocytes more resistant to the blockade effect of anti--CD11a (LFA-1) and, as result, may alter killing of tumour target cells in vitro.
L Hoffman-Goetz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  15     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    1995 Sep-Oct
Date Detail:
Created Date:  1996-03-01     Completed Date:  1996-03-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  GREECE    
Other Details:
Languages:  eng     Pagination:  1981-4     Citation Subset:  IM    
Department of Health Studies and Gerontology, Faculty of Applied Health Sciences, University of Waterloo, Ontario, Canada.
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MeSH Terms
Antibodies, Monoclonal / immunology
Cytotoxicity, Immunologic
Interleukin-2 / pharmacology
Killer Cells, Natural / chemistry,  immunology*
Lymphocyte Function-Associated Antigen-1 / analysis*,  immunology,  physiology
Mice, Inbred C3H
Physical Conditioning, Animal*
Spleen / cytology
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Interleukin-2; 0/Lymphocyte Function-Associated Antigen-1

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