| Effect of acute streptozotocin diabetes on fatty acid content and composition in different lipid fractions of rat skeletal muscle. | |
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MedLine Citation:
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PMID: 10333079 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of the present study was to examine the effect of acute streptozotocin diabetes on long chain fatty acid content and composition in different lipid classes of particular muscle types in the rat. Two days after streptozotocin administration, rats were anesthetised, and the white and red sections of the gastrocnemius, the soleus and the blood were taken. Lipids were extracted with chloroform/methanol and separated into different fractions (phospholipids, free fatty acids, di- and triacylglycerols) by means of thin layer chromatography. Fatty acids of each fraction were identified and quantified by means of gas-liquid chromatography. The diabetes resulted in elevation of the concentration of blood glucose (over four-fold) and the plasma free fatty acid (over two-fold). Total free fatty acid content in the muscles of diabetic rats increased by 26% in the white, 24% in the red gastrocnemius and 21% in the soleus. There were also changes in the composition of that fraction in each muscle. Diacylglycerol fatty acid content was elevated in both parts of the gastrocnemius (the white part by 15%, the red part by 44%) and remained stable in the soleus of the diabetic rats. The content of triacylglycerol fatty acids was elevated only in the red gastrocnemius in the diabetic group (by 112%), but changes in fatty acid composition in this fraction occurred in each muscle. The content of phospholipid fatty acids was elevated in the white gastrocnemius (by 13%) and remained stable in other muscles. There were only minor changes in phospholipid fatty acid composition in the diabetic rats. We concluded that acute insulin deficiency changes fatty acid content and composition in skeletal muscle lipids. The changes depend both on lipid fraction and muscle type. |
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Authors:
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A Nawrocki; M Górska; M Zendzian-Piotrowska; J Górski |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et métabolisme Volume: 31 ISSN: 0018-5043 ISO Abbreviation: Horm. Metab. Res. Publication Date: 1999 Apr |
Date Detail:
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Created Date: 1999-07-01 Completed Date: 1999-07-01 Revised Date: 2009-02-19 |
Medline Journal Info:
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Nlm Unique ID: 0177722 Medline TA: Horm Metab Res Country: GERMANY |
Other Details:
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Languages: eng Pagination: 252-6 Citation Subset: IM |
Affiliation:
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Department of Physiology, Medical School of Bialystok, Poland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Glucose / metabolism Chromatography, Thin Layer Diabetes Mellitus, Experimental / blood, metabolism* Diglycerides / analysis Fatty Acids / analysis* Fatty Acids, Nonesterified / analysis, blood Lipids / analysis* Male Muscle, Skeletal / chemistry* Phospholipids / analysis Rats Rats, Wistar Triglycerides / analysis |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 0/Diglycerides; 0/Fatty Acids; 0/Fatty Acids, Nonesterified; 0/Lipids; 0/Phospholipids; 0/Triglycerides |
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