Document Detail

Effect of acute exercise on endothelial progenitor cells in patients with peripheral arterial disease.
MedLine Citation:
PMID:  17390544     Owner:  NLM     Status:  MEDLINE    
To determine whether exercise increases endothelial progenitor cells (EPCs) in patients with peripheral vascular disease, we developed a multi-parameter flow cytometry assay to rigorously assess EPCs and mature endothelial cells (ECs) in control subjects and patients with peripheral artery disease (PAD) subjected to graded exercise. Blood was collected from young healthy subjects (n = 9, mean age 33 years), older healthy subjects (n = 13, mean age 66 years), and older subjects with PAD (n = 15, mean age 69 years) before and 10 minutes after exercise. White blood cells were isolated and stained with a five-color antibody panel: FITC-anti-CD31, PE-anti-CD146, PE-anti-CD133, PerCP-Cy5.5-anti-CD3,-CD19,-CD33, PE-Cy7-anti-CD34, and APC-anti-VEGF-R2. Viability was assessed by propidium iodide exclusion. Viable, low, side scatter singlets that were CD3-, 19-, and 33-negative were counted. While baseline levels of EPCs and ECs were similar among all subjects, young healthy subjects demonstrated significantly greater (p < 0.05) levels of progenitor cells (PCs) than older healthy and PAD subjects. Levels of EPCs and ECs tended to increase in all subjects after exercise; however, increases in PCs were only observed in young healthy and PAD subjects. Further, trends in the magnitude of change of subsets with exercise were most similar between young and PAD subjects. Our findings suggest that aging may reduce baseline circulating levels of PCs, but not EPCs or ECs, and that exercise-induced mobilization of subsets may differ depending on age and presence of PAD.
Rebecca Gusic Shaffer; Sam Greene; Arash Arshi; Greg Supple; Andrew Bantly; Jonni S Moore; Michael S Parmacek; Emile R Mohler
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Vascular medicine (London, England)     Volume:  11     ISSN:  1358-863X     ISO Abbreviation:  Vasc Med     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2007-03-29     Completed Date:  2007-04-20     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9610930     Medline TA:  Vasc Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  219-26     Citation Subset:  IM    
Department of Medicine, Cardiovascular Division, Flow Cytometry and Cell Sorting Resource Laboratory, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
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MeSH Terms
Aging / blood*,  pathology
Antigens, CD / blood
Cell Differentiation
Cell Lineage
Cell Shape
Cells, Cultured
Colony-Forming Units Assay
Endothelial Cells / pathology*
Flow Cytometry / methods
Intermittent Claudication / blood*,  etiology,  pathology
Lectins / blood
Lipoproteins, LDL / blood
Peripheral Vascular Diseases / blood*,  complications,  pathology
Stem Cells / pathology*
Vascular Endothelial Growth Factor Receptor-2 / blood
von Willebrand Factor / analysis
Reg. No./Substance:
0/Antigens, CD; 0/Lectins; 0/Lipoproteins, LDL; 0/acetyl-LDL; 0/von Willebrand Factor; EC Endothelial Growth Factor Receptor-2
Comment In:
Vasc Med. 2006 Nov;11(4):258   [PMID:  17390550 ]

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