| Effect of activated human polymorphonuclear leucocytes on T lymphocyte proliferation and viability. | |
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MedLine Citation:
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PMID: 23025756 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human polymorphonuclear leucocytes (PMN) are thought to be immunosuppressive. The suppressive mechanism(s) used by PMN are, however, not well defined and in this study they were analysed using T-cell responses to CD3(+) CD28 monoclonal antibodies (mAb) as a readout. We demonstrate that in vitro activated PMN (PMN(act)) can, without any T-cell interaction, induce apparent T-cell suppression by inhibiting the stimulatory capacity of the CD3 mAb. However, a cell-directed suppression of T-cell proliferation was observed when PMN(act) were added to pre-activated T cells that are already committed to polyclonal proliferation. This suppression was partially reversed by catalase addition (P < 0·01) and largely reversed by addition of exogenous interleukin-2 (P < 0·001) but was not significantly reduced by nitric oxide synthase inhibition, myeloperoxidase inhibition or addition of excess arginine. Following removal of PMN(act) , suppressed T cells could respond normally to further stimulation. In addition to suppressing proliferation, co-culture with PMN(act) also induced a significant decrease in T-cell viability that was reversed by catalase addition (P < 0·05). The addition of the arginase inhibitor N-hydroxy-nor-l-arginine induced both a further significant, catalase-sensitive, loss in T-cell viability and increased nitrite release (P < 0·001). These data demonstrate that PMN, when activated, can both induce T-cell death and reversibly inhibit proliferation of activated T cells. The mechanisms underlying these distinct processes and the effects of arginase inhibitors on PMN induced cytotoxicity merit further investigation. |
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Authors:
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Barry D Hock; Karen G Taylor; Nicholas B Cross; Anthony J Kettle; Mark B Hampton; Judith L McKenzie |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Immunology Volume: 137 ISSN: 1365-2567 ISO Abbreviation: Immunology Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-10-02 Completed Date: 2012-12-03 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 0374672 Medline TA: Immunology Country: England |
Other Details:
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Languages: eng Pagination: 249-58 Citation Subset: IM |
Copyright Information:
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© 2012 The Authors. Immunology © 2012 Blackwell Publishing Ltd. |
Affiliation:
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Haematology Research Group, Christchurch Hospital, Christchurch, New Zealand. barry.hock@otago.ac.nz |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Proliferation* Cell Survival Cells, Cultured Coculture Techniques Humans Lymphocyte Activation Neutrophils / cytology, immunology* T-Lymphocytes / cytology, immunology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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