Document Detail

Effect of activated human polymorphonuclear leucocytes on T lymphocyte proliferation and viability.
MedLine Citation:
PMID:  23025756     Owner:  NLM     Status:  MEDLINE    
Human polymorphonuclear leucocytes (PMN) are thought to be immunosuppressive. The suppressive mechanism(s) used by PMN are, however, not well defined and in this study they were analysed using T-cell responses to CD3(+) CD28 monoclonal antibodies (mAb) as a readout. We demonstrate that in vitro activated PMN (PMN(act)) can, without any T-cell interaction, induce apparent T-cell suppression by inhibiting the stimulatory capacity of the CD3 mAb. However, a cell-directed suppression of T-cell proliferation was observed when PMN(act) were added to pre-activated T cells that are already committed to polyclonal proliferation. This suppression was partially reversed by catalase addition (P < 0·01) and largely reversed by addition of exogenous interleukin-2 (P < 0·001) but was not significantly reduced by nitric oxide synthase inhibition, myeloperoxidase inhibition or addition of excess arginine. Following removal of PMN(act) , suppressed T cells could respond normally to further stimulation. In addition to suppressing proliferation, co-culture with PMN(act) also induced a significant decrease in T-cell viability that was reversed by catalase addition (P < 0·05). The addition of the arginase inhibitor N-hydroxy-nor-l-arginine induced both a further significant, catalase-sensitive, loss in T-cell viability and increased nitrite release (P < 0·001). These data demonstrate that PMN, when activated, can both induce T-cell death and reversibly inhibit proliferation of activated T cells. The mechanisms underlying these distinct processes and the effects of arginase inhibitors on PMN induced cytotoxicity merit further investigation.
Barry D Hock; Karen G Taylor; Nicholas B Cross; Anthony J Kettle; Mark B Hampton; Judith L McKenzie
Related Documents :
24622326 - Total body irradiation causes long-term mouse bm injury via induction of hsc premature ...
23109106 - Selective cell recruitment and spatially controlled cell attachment on instructive chit...
22936956 - Correction: sa-4-1bbl costimulation inhibits conversion of conventional cd4(+) t cells ...
24084446 - Cytokine induced killer (cik) cells for the treatment of haematological neoplasms.
16382886 - Nonhematopoietic stem cells of fetal origin--how much of today's enthusiasm will pass t...
15710566 - Bone marrow stromal cells transduced with human hemangiopoietin gene support hematopoie...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology     Volume:  137     ISSN:  1365-2567     ISO Abbreviation:  Immunology     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-02     Completed Date:  2012-12-03     Revised Date:  2013-11-05    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  England    
Other Details:
Languages:  eng     Pagination:  249-58     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors. Immunology © 2012 Blackwell Publishing Ltd.
Haematology Research Group, Christchurch Hospital, Christchurch, New Zealand.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Proliferation*
Cell Survival
Cells, Cultured
Coculture Techniques
Lymphocyte Activation
Neutrophils / cytology,  immunology*
T-Lymphocytes / cytology,  immunology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Isolation of human MHC class II-restricted T cell receptors from the autologous T-cell repertoire wi...
Next Document:  Antitussive effects of the peripherally restricted GABAB receptor agonist lesogaberan in guinea pigs...