Document Detail


Effect of actin cytoskeleton disruption on electric pulse-induced apoptosis and electroporation in tumour cells.
MedLine Citation:
PMID:  20828369     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Electric pulses are known to affect the outer membrane and intracellular structures of tumour cells. By applying electrical pulses of 450 ns duration with electric field intensity of 8 kV/cm to HepG2 cells for 30 s, electric pulse-induced changes in the integrity of the plasma membrane, apoptosis, viability and mitochondrial transmembrane potential were investigated. Results demonstrated that electric pulses induced cell apoptosis and necrosis accompanied with the decrease of mitochondrial transmembrane potential and the formation of pores in the membrane. The role of cytoskeleton in cellular response to electric pulses was investigated. We found that the apoptotic and necrosis percentages of cells in response to electric pulses decreased after cytoskeletal disruption. The electroporation of cell was not affected by cytoskeletal disruption. The results suggest that the disruption of actin skeleton is positive in protecting cells from killing by electric pulses, and the skeleton is not involved in the electroporation directly.
Authors:
Deyou Xiao; Liling Tang; Chao Zeng; Jianfei Wang; Xiao Luo; Chenguo Yao; Caixin Sun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  35     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-25     Completed Date:  2011-05-03     Revised Date:  2012-08-02    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  99-104     Citation Subset:  IM    
Affiliation:
Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, Peoples Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Actin Cytoskeleton / metabolism,  physiology*
Apoptosis*
Cell Survival
Electroporation*
Hep G2 Cells
Humans
Membrane Potential, Mitochondrial / physiology
Time Factors

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