| Effect of actin cytoskeleton disruption on electric pulse-induced apoptosis and electroporation in tumour cells. | |
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MedLine Citation:
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PMID: 20828369 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Electric pulses are known to affect the outer membrane and intracellular structures of tumour cells. By applying electrical pulses of 450 ns duration with electric field intensity of 8 kV/cm to HepG2 cells for 30 s, electric pulse-induced changes in the integrity of the plasma membrane, apoptosis, viability and mitochondrial transmembrane potential were investigated. Results demonstrated that electric pulses induced cell apoptosis and necrosis accompanied with the decrease of mitochondrial transmembrane potential and the formation of pores in the membrane. The role of cytoskeleton in cellular response to electric pulses was investigated. We found that the apoptotic and necrosis percentages of cells in response to electric pulses decreased after cytoskeletal disruption. The electroporation of cell was not affected by cytoskeletal disruption. The results suggest that the disruption of actin skeleton is positive in protecting cells from killing by electric pulses, and the skeleton is not involved in the electroporation directly. |
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Authors:
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Deyou Xiao; Liling Tang; Chao Zeng; Jianfei Wang; Xiao Luo; Chenguo Yao; Caixin Sun |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell biology international Volume: 35 ISSN: 1095-8355 ISO Abbreviation: Cell Biol. Int. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-25 Completed Date: 2011-05-03 Revised Date: 2012-08-02 |
Medline Journal Info:
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Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: England |
Other Details:
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Languages: eng Pagination: 99-104 Citation Subset: IM |
Affiliation:
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Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, Peoples Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actin Cytoskeleton
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metabolism,
physiology* Apoptosis* Cell Survival Electroporation* Hep G2 Cells Humans Membrane Potential, Mitochondrial / physiology Time Factors |
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