Document Detail

Effect of actin cytoskeleton disruption on electric pulse-induced apoptosis and electroporation in tumour cells.
MedLine Citation:
PMID:  20828369     Owner:  NLM     Status:  MEDLINE    
Electric pulses are known to affect the outer membrane and intracellular structures of tumour cells. By applying electrical pulses of 450 ns duration with electric field intensity of 8 kV/cm to HepG2 cells for 30 s, electric pulse-induced changes in the integrity of the plasma membrane, apoptosis, viability and mitochondrial transmembrane potential were investigated. Results demonstrated that electric pulses induced cell apoptosis and necrosis accompanied with the decrease of mitochondrial transmembrane potential and the formation of pores in the membrane. The role of cytoskeleton in cellular response to electric pulses was investigated. We found that the apoptotic and necrosis percentages of cells in response to electric pulses decreased after cytoskeletal disruption. The electroporation of cell was not affected by cytoskeletal disruption. The results suggest that the disruption of actin skeleton is positive in protecting cells from killing by electric pulses, and the skeleton is not involved in the electroporation directly.
Deyou Xiao; Liling Tang; Chao Zeng; Jianfei Wang; Xiao Luo; Chenguo Yao; Caixin Sun
Related Documents :
20801029 - Affibody-displaying bionanocapsules for specific drug delivery to her2-expressing cance...
18331509 - Low-frequency electromagnetic field effects on functional groups in human skin keratino...
18504979 - Whole cell electrochemistry of electricity-producing microorganisms evidence an adaptat...
9837779 - Enhanced proliferation caused by a low frequency weak magnetic field in chick embryo fi...
19107119 - Selective enhancement of the uptake and bioactivity of a tat-conjugated peptide inhibit...
16349089 - Electrochemical prevention of marine biofouling with a carbon-chloroprene sheet.
4632309 - Mechanism for the regulation of cell division in agmenellum.
1328069 - Progressive abrogation of tgf-beta 1 and egf growth control is associated with tumour p...
18202789 - Protective effects of resveratrol on uvb-irradiated hacat cells through attenuation of ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  35     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-25     Completed Date:  2011-05-03     Revised Date:  2012-08-02    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  99-104     Citation Subset:  IM    
Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, Peoples Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Actin Cytoskeleton / metabolism,  physiology*
Cell Survival
Hep G2 Cells
Membrane Potential, Mitochondrial / physiology
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  MicroRNA-488 suppresses cell migration through modulation of the focal adhesion activity during chon...
Next Document:  Metallothionein in human immunomagnetically selected CD34+ haematopoietic progenitor cells.