| Effect of acidosis on IL-8 and MCP-1 during hypoxia and reoxygenation in human NT2-N neurons. | |
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MedLine Citation:
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PMID: 16919250 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Inflammation probably plays a significant role in perinatal brain injury. To study the contribution of locally produced cytokines, the effect on cell death of addition of IL-8 and MCP-1 or antibodies to these, and the impact of acidosis, human postmitotic NT2-N neurons were exposed to 3 h of hypoxia and glucose deprivation and reoxygenated for 21 h. After 3 h of hypoxia with neutral medium, IL-8 was significantly increased compared to controls (150 (100-250)% vs. 100 (85-115)%, p=0.023). After 21 h of neutral reoxygenation, both IL-8 (380 (110-710)% vs. 150 (85-260)%, p=0.041) and monocyte chemoattractant protein-1 (MCP-1) (650 (440-2000)% vs. 310 (230-340)%, p=0.007) were significantly increased compared to controls. After 3 h of hypoxia, both IL-8 (p=0.002) and MCP-1 (p=0.008) were significantly lower in cells with acidotic compared with cells with neutral medium. Acidosis during reoxygenation, however, significantly increased IL-8 release, whereas MCP-1 release was diminished. Similar effects of acidosis were seen in normoxic controls. The cells also secreted RANTES and IP-10, but not 8 other cytokines tested. We found no effect on cell death, measured by MTT assay, of addition of IL-8, MCP-1 or antibodies to these. We conclude that human NT2-N neurons release IL-8 and MCP-1 during 21 h of reoxygenation after 3 h of hypoxia. Acidosis led to a differential effect on IL-8 and MCP-1, with increased IL-8 and decreased MCP-1, both during reoxygenation and in normoxic controls. IL-8 and MCP-1 had no effect on cell death. |
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Authors:
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Elisabeth Frøyland; Elena Didenko Pedersen; Anne-Katrine Kvissel; Runar Almaas; Anne Pharo; Bjørn Steen Skålhegg; Tom Eirik Mollnes; Terje Rootwelt |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-08-17 |
Journal Detail:
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Title: Brain research Volume: 1113 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-09-25 Completed Date: 2006-12-13 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 64-73 Citation Subset: IM |
Affiliation:
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Department of Pediatric Research, Rikshospitalet-Radiumhospitalet Medical Center and University of Oslo, N-0027 Oslo, Norway. elisabeth.froyland@medisin.uio.no |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acidosis
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metabolism* Anoxia Antibodies / pharmacology Cell Hypoxia / physiology Cell Line Chemokine CCL2 / immunology, metabolism* Chemokine CCL5 / metabolism Dose-Response Relationship, Drug Fluorescent Antibody Technique / methods Gene Expression Regulation / drug effects* Glucose / deficiency Humans Interleukin-8 / immunology, metabolism* Neurofilament Proteins / metabolism Neurons / physiology* Oxygen / administration & dosage* Statistics, Nonparametric Tetrazolium Salts / diagnostic use Thiazoles / diagnostic use Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Chemokine CCL2; 0/Chemokine CCL5; 0/Interleukin-8; 0/Neurofilament Proteins; 0/Tetrazolium Salts; 0/Thiazoles; 108688-71-7/neurofilament protein H; 298-93-1/thiazolyl blue; 50-99-7/Glucose; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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