Document Detail

Effect of Ultrasound Irradiation on α-SMA and TGF-β1 Expression in Human Dermal Fibroblasts.
MedLine Citation:
PMID:  21937873     Owner:  NLM     Status:  In-Data-Review    
Ultrasound therapy is used to promote pressure ulcer healing as an adjunctive therapy. However, the efficacy and the scientific basis of this treatment are unclear. We investigated the effect of ultrasound irradiation on alpha-smooth muscle actin (α-SMA) and transforming growth factor-beta1 (TGF-β1) expression in human dermal fibroblasts. These are important factors for acceleration of wound closure. We used pulsed ultrasound of 0, 0.1, 0.5, and 1.0 W/cm2. TGF-β1 and α-SMA mRNA was measured by quantitative real-time polymerase chain reaction, α-SMA protein was examined by western blot, and localization of α-SMA was evaluated by immunofluorescence staining. Expression of α-SMA and TGF-β1 mRNA was increased at 24 h but not at 48 h after ultrasound irradiation. There were significant differences between controls of 0 W/cm2 and 0.1 W/cm2 with a 1.34 ± 0.26 fold increase in α-SMA (P < 0.05) and a 1.78 ± 0.57 fold increase in TGF-β1 (P < 0.05). Protein levels of α-SMA were also increased and detected in ultrasound irradiated fibroblasts at 24 h. Ultrasound irradiation promotes α-SMA expression in human dermal fibroblasts and this suggests the biological mechanism of ultrasound efficacy on chronic wound treatment.
Noriaki Maeshige; Hiroto Terashi; Michiko Aoyama; Kazuhiro Torii; Masaharu Sugimoto; Makoto Usami
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Publication Detail:
Type:  Journal Article     Date:  2011-05-11
Journal Detail:
Title:  The Kobe journal of medical sciences     Volume:  56     ISSN:  1883-0498     ISO Abbreviation:  Kobe J Med Sci     Publication Date:  2011  
Date Detail:
Created Date:  2011-09-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0413531     Medline TA:  Kobe J Med Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  E242-52     Citation Subset:  IM    
Division of Nutrition and Metabolism, Department of Biophysics, Kobe University Graduate School of Health Sciences, 7-10-2 Tomogaoka, Suma-ku, Kobe 654-0142, Japan.
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