Document Detail

Effect of Ultra High Hydrostatic Pressure on the Activity and Structure of Mushroom (Agaricus bisporus) Polyphenoloxidase.
MedLine Citation:
PMID:  22136649     Owner:  NLM     Status:  Publisher    
High hydrostatic pressure (HHP, treatment pressure≤700 MPa) is approved to be the most successful commercial non-thermal processing due to its minimal modifications in nutritional and sensory quality. However, for some pressure stable enzymes such as PPO, this unique technology can hardly inactivate them at treatment pressure below of 700 MPa. This study investigated the effects of ultra high hydrostatic pressure (UHHP, treatment pressure>700 MPa) on the activity of Agaricus bisporus mushroom polyphenoloxidase (PPO) both in the phosphate buffer and in the mushroom puree, and on the structure of the enzyme by means of circular dichroism (CD), fluorescence emission spectra and sulphydryl groups detection. The results showed that UHHP treatment at pressure from 800 to 1600 MPa caused significant inactivation on the PPO both in the phosphate buffer and in the mushroom puree. UHHP treatment at 1400 MPa and 1600 MPa for 1 min reduced the enzyme activity by 90.4% and 99.2% in the buffer, however, higher enzyme activity remained in the puree after UHHP treatment at the same condition. CD and fluorescence spectra analysis showed that the secondary and tertiary structures of UHHP treated mushroom PPO were changed. The sulphydryl groups (SH) detection revealed that the SH content on the surface of UHHP treated mushroom PPO was increased. It has been suggested that the inactivation of mushroom PPO by UHHP treatment at pressure higher than 1000 MPa was due to the synergistic effect of the pressure and the heat arising from pressurization, in which the heat play a major role.
Jianyong Yi; Bin Jiang; Zhong Zhang; Xiaojun Liao; Yan Zhang; Xiaosong Hu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-4
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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