| Effect of Tween Series on Growth and cis-9, trans-11 Conjugated Linoleic Acid Production of Lactobacillus acidophilus F0221 in the Presence of Bile Salts. | |
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MedLine Citation:
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PMID: 22272124 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Cis-9, trans-11 conjugated linoleic acid (c9, t11 CLA) producing bacteria have attracted much attention as novel probiotics which have shown beneficial effects on host health. However, bile salts are able to inhibit bacterial growth and c9, t11 CLA production. For recovering growth and c9, t11 CLA production of Lactobacillus acidophilus F0221 in the presence of bile salts, Tween series (Tween 20, Tween 40, Tween 60 and Tween 80) were added in growth culture containing 0.3% oxgall. Results showed that the viable counts were significantly (P < 0.05) recovered to 8.58-8.75 log CFU/mL in the presence of all Tween treatments. However, recovery of c9, t11 CLA production was only demonstrated in the presence of Tween 80 (72.89 μg/mL). Stepwise increasing oxgall in a concentrations range from 0.1% to 0.9% according to human intestinal physiological environments, Tween 80 still showed significant (P < 0.05) recovery ability on growth (8.91-8.04 log CFU/mL) and c9, t11 CLA (69.22-34.27 μg/mL) production. The effect of Tween 80 on growth and production was also investigated in the presence of different types of bile salts (sodium salts of cholic acid (CA), deoxycholic acid (DCA), chendeoxycholic acid (CDCA), glycocholic acid (GCA) and taurocholic acid (TCA)). Results showed that Tween 80 could significantly (P < 0.05) recover c9, t11 CLA production in the presence of all types of bile salts, but the Tween 80 could only significantly (P < 0.05) recover viable counts of the strain in the presence of CA, DCA and CDCA. This recovery ability could be attributed to the protection of leakage of intracellular material. Additionally, although bile salts inhibited growth and c9, t11 CLA production by the growing cell, it promoted the c9, t11 CLA production by the resting cell. |
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Authors:
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Jing-Yan Li; Lan-Wei Zhang; Ming Du; Xue Han; Hua-Xi Yi; Chun-Feng Guo; Ying-Chun Zhang; Xue Luo; Yan-He Zhang; Yu-Juan Shan; Ai-Ju Hou |
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Publication Detail:
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Type: Journal Article Date: 2011-12-08 |
Journal Detail:
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Title: International journal of molecular sciences Volume: 12 ISSN: 1422-0067 ISO Abbreviation: Int J Mol Sci Publication Date: 2011 |
Date Detail:
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Created Date: 2012-01-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101092791 Medline TA: Int J Mol Sci Country: Switzerland |
Other Details:
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Languages: eng Pagination: 9138-54 Citation Subset: - |
Affiliation:
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School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China; E-Mails: jyli@hit.edu.cn (J.-Y.L.); duming121@163.com (M.D.); jiayuanhan@yahoo.com.cn (X.H.); yihx2006@yahoo.com.cn (H.-X.Y.); zyc229@163.com (Y.-C.Z.); luoxue1984@126.com (X.L.); yujuan72@163.com (Y.-J.S.); aijuhellea@yahoo.com.cn (A.-J.H.). |
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