| Effect of Tunisian Capparis spinosa L. extract on melanogenesis in B16 murine melanoma cells. | |
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MedLine Citation:
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PMID: 19685105 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of Tunisian Capparis spinosa L. aromatic plant extract on melanogenesis regulation in B16 murine melanoma cells was investigated. B16 cells were treated with 0.0005, 0.005, and 0.05% (w/v) C. spinosa extract after which the melanin content and cell viability were measured. To clarify the mechanism behind melanogenesis regulation, the expression of tyrosinase was determined. Results showed that the extract had a significant stimulative effect on melanogenesis in B16 cells in a dose-dependent manner without cytotoxicity. Western blot analysis showed that expression of tyrosinase in cells treated with 0.03% (w/v) C. spinosa extract increased by 12.5- and 20-fold after 24 and 48 h of incubation, respectively, compared with untreated cells. HPLC analysis of the extract revealed the presence of 1% quercetin, a known melanogenesis stimulator, indicating that our findings may be attributed to quercetin; however, other compounds present in the extract may also have an effect on the overall ability of the extract to stimulate melanogenesis. We report here that Tunisian C. spinosa leaf extract can stimulate melanogenesis in a dose-dependent manner without cytotoxicity by increasing tyrosinase protein expression and has the potential to be used as a possible tanning agent or as a treatment for hair depigmentation. |
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Authors:
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Kyoko Matsuyama; Myra O Villareal; Abdelfatteh El Omri; Junkyu Han; Mohamed Elyes Kchouk; Hiroko Isoda |
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Publication Detail:
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Type: Journal Article Date: 2009-08-15 |
Journal Detail:
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Title: Journal of natural medicines Volume: 63 ISSN: 1861-0293 ISO Abbreviation: J Nat Med Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-09-04 Completed Date: 2010-01-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101518405 Medline TA: J Nat Med Country: Japan |
Other Details:
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Languages: eng Pagination: 468-72 Citation Subset: IM |
Affiliation:
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Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Capparis / chemistry* Cell Line, Tumor Cell Survival / drug effects Chromatography, High Pressure Liquid Gene Expression Regulation, Enzymologic / drug effects* Melanins / biosynthesis* Melanoma, Experimental Mice Monophenol Monooxygenase / metabolism Plant Extracts / pharmacology* Tunisia |
| Chemical | |
Reg. No./Substance:
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0/Melanins; 0/Plant Extracts; EC 1.14.18.1/Monophenol Monooxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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