Document Detail


Effect of Tunisian Capparis spinosa L. extract on melanogenesis in B16 murine melanoma cells.
MedLine Citation:
PMID:  19685105     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of Tunisian Capparis spinosa L. aromatic plant extract on melanogenesis regulation in B16 murine melanoma cells was investigated. B16 cells were treated with 0.0005, 0.005, and 0.05% (w/v) C. spinosa extract after which the melanin content and cell viability were measured. To clarify the mechanism behind melanogenesis regulation, the expression of tyrosinase was determined. Results showed that the extract had a significant stimulative effect on melanogenesis in B16 cells in a dose-dependent manner without cytotoxicity. Western blot analysis showed that expression of tyrosinase in cells treated with 0.03% (w/v) C. spinosa extract increased by 12.5- and 20-fold after 24 and 48 h of incubation, respectively, compared with untreated cells. HPLC analysis of the extract revealed the presence of 1% quercetin, a known melanogenesis stimulator, indicating that our findings may be attributed to quercetin; however, other compounds present in the extract may also have an effect on the overall ability of the extract to stimulate melanogenesis. We report here that Tunisian C. spinosa leaf extract can stimulate melanogenesis in a dose-dependent manner without cytotoxicity by increasing tyrosinase protein expression and has the potential to be used as a possible tanning agent or as a treatment for hair depigmentation.
Authors:
Kyoko Matsuyama; Myra O Villareal; Abdelfatteh El Omri; Junkyu Han; Mohamed Elyes Kchouk; Hiroko Isoda
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Publication Detail:
Type:  Journal Article     Date:  2009-08-15
Journal Detail:
Title:  Journal of natural medicines     Volume:  63     ISSN:  1861-0293     ISO Abbreviation:  J Nat Med     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-09-04     Completed Date:  2010-01-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101518405     Medline TA:  J Nat Med     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  468-72     Citation Subset:  IM    
Affiliation:
Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Capparis / chemistry*
Cell Line, Tumor
Cell Survival / drug effects
Chromatography, High Pressure Liquid
Gene Expression Regulation, Enzymologic / drug effects*
Melanins / biosynthesis*
Melanoma, Experimental
Mice
Monophenol Monooxygenase / metabolism
Plant Extracts / pharmacology*
Tunisia
Chemical
Reg. No./Substance:
0/Melanins; 0/Plant Extracts; EC 1.14.18.1/Monophenol Monooxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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