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Effect of Trolox addition to cryopreservation media on human sperm motility.
MedLine Citation:
PMID:  25242981     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Sperm parameters and motion kinetics are affected by cryopreservation.
OBJECTIVE: The main purpose of the current study was to determine the effect of different concentrations of Trolox as an antioxidant to freezing-thawing procedure on human sperm kinematic parameter.
MATERIALS AND METHODS: Semen was collected from 20 normal donors and divided into five aliquots prior to cryopreservation. The first aliquot was analyzed by computer-assisted sperm analysis (CASA). Other aliquots were mixed with cryo-protective agent containing 0, 20, 40, and 80 µmol Trolox and treated samples were cryopreserved in liquid nitrogen. After two weeks samples were thawed and sperm motion kinematics was measured by CASA. Percent motility (Mot), curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), linearity (LIN), and amplitude of lateral head displacement (ALH) were compared before and after freeze.
RESULTS: Addition of 40µmol Trolox resulted in significantly higher (p<0.05) post thaw VCL, VSL and VAP compared to other groups. Therefore the percentage of post thaw motile spermatozoa were significantly higher (p<0.01).
CONCLUSION: The supplementation of Trolox significantly improved the post-thawed human semen quality, especially progressive motility and average path velocity.
Authors:
Mohammad Baqer Minaei; Mohammad Barbarestani; Saeid Nekoonam; Mir Abbas Abdolvahabi; Nasrin Takzare; Mohammad Hossein Asadi; Azim Hedayatpour; Fardin Amidi
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Iranian journal of reproductive medicine     Volume:  10     ISSN:  1680-6433     ISO Abbreviation:  Iran J Reprod Med     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2014-09-22     Completed Date:  2014-09-22     Revised Date:  2014-10-03    
Medline Journal Info:
Nlm Unique ID:  101463749     Medline TA:  Iran J Reprod Med     Country:  Iran    
Other Details:
Languages:  eng     Pagination:  99-104     Citation Subset:  -    
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