Document Detail

Effect of TGF-beta/Smad signaling on sertoli cell and possible mechanism related to complete sertoli cell-only syndrome.
MedLine Citation:
PMID:  18648910     Owner:  NLM     Status:  MEDLINE    
The roles of TGF-beta and the interaction between TGF-beta and EGFR signaling are critical in Sertoli cell, though the knowledge about them is limited. RT-PCR was used to characterize the status of TGF-beta signaling in clinical testicular specimens with complete Sertoli cell-only syndrome (SCOS). The mouse Sertoli cell TM4 was used to investigate the interaction between TGF-beta and EGFR signaling by using mitogenic assay, luciferase assay, and western blot, while TM3 (mouse leydig cell), 3T3 (mouse embryo fibroblasts), and B82 (mouse lung fibroblasts) were selected as control. The RT-PCR assay indicated that the expression levels of TbetaRII and Smad2 in SCOS testes were upregulated compared to that in the normal controls. In the in vitro experiment, the TGF-beta1 downregulated cellular proliferation of TM3 and B82 cell (P < 0.05), but it did not changed the proliferation of TM4 and 3T3 cells (P > 0.05). On contrast, TGF-beta1 only increased the TGF response elements p3TP-lux activity significantly (P < 0.05) in Sertoli cell TM4. Also, the Western blot assay shows an obvious increase of Smad2 in TM4, 3T3, and TM3 cells after TGF-beta1 treatment while the EGFR expression level was significantly increased in TM4 cells only. In conclusion, the TGF-beta pathway and the cross-link between TGF-beta and EGFR signaling may play an important role on the dysfunction of Sertoli cells which induce germ stem cells' disappearance in SCOS.
Tao Sun; Zhongcheng Xin; Zhe Jin; Yiguang Wu; Yanqing Gong
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-22
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  319     ISSN:  1573-4919     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-10-22     Completed Date:  2009-06-16     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1-7     Citation Subset:  IM    
Andrology Center, Peking University First Hospital, Peking University, Xicheng District, Beijing, People's Republic of China.
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MeSH Terms
3T3 Cells
Azoospermia / metabolism,  pathology
Gene Expression Regulation / drug effects
Receptor, Epidermal Growth Factor / biosynthesis
Receptors, Transforming Growth Factor beta / metabolism*
Sertoli Cell-Only Syndrome / metabolism*,  pathology
Sertoli Cells / metabolism*,  pathology
Signal Transduction*
Smad2 Protein / metabolism*
Transforming Growth Factor beta1 / metabolism*,  pharmacology
Reg. No./Substance:
0/Receptors, Transforming Growth Factor beta; 0/SMAD2 protein, human; 0/Smad2 Protein; 0/Smad2 protein, mouse; 0/Transforming Growth Factor beta1; EC protein, human; EC, Epidermal Growth Factor

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