| Effect of TGF-beta/Smad signaling on sertoli cell and possible mechanism related to complete sertoli cell-only syndrome. | |
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MedLine Citation:
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PMID: 18648910 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The roles of TGF-beta and the interaction between TGF-beta and EGFR signaling are critical in Sertoli cell, though the knowledge about them is limited. RT-PCR was used to characterize the status of TGF-beta signaling in clinical testicular specimens with complete Sertoli cell-only syndrome (SCOS). The mouse Sertoli cell TM4 was used to investigate the interaction between TGF-beta and EGFR signaling by using mitogenic assay, luciferase assay, and western blot, while TM3 (mouse leydig cell), 3T3 (mouse embryo fibroblasts), and B82 (mouse lung fibroblasts) were selected as control. The RT-PCR assay indicated that the expression levels of TbetaRII and Smad2 in SCOS testes were upregulated compared to that in the normal controls. In the in vitro experiment, the TGF-beta1 downregulated cellular proliferation of TM3 and B82 cell (P < 0.05), but it did not changed the proliferation of TM4 and 3T3 cells (P > 0.05). On contrast, TGF-beta1 only increased the TGF response elements p3TP-lux activity significantly (P < 0.05) in Sertoli cell TM4. Also, the Western blot assay shows an obvious increase of Smad2 in TM4, 3T3, and TM3 cells after TGF-beta1 treatment while the EGFR expression level was significantly increased in TM4 cells only. In conclusion, the TGF-beta pathway and the cross-link between TGF-beta and EGFR signaling may play an important role on the dysfunction of Sertoli cells which induce germ stem cells' disappearance in SCOS. |
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Authors:
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Tao Sun; Zhongcheng Xin; Zhe Jin; Yiguang Wu; Yanqing Gong |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-07-22 |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 319 ISSN: 1573-4919 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-10-22 Completed Date: 2009-06-16 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1-7 Citation Subset: IM |
Affiliation:
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Andrology Center, Peking University First Hospital, Peking University, Xicheng District, Beijing, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Adult Animals Azoospermia / metabolism, pathology Gene Expression Regulation / drug effects Humans Male Mice Receptor, Epidermal Growth Factor / biosynthesis Receptors, Transforming Growth Factor beta / metabolism* Sertoli Cell-Only Syndrome / metabolism*, pathology Sertoli Cells / metabolism*, pathology Signal Transduction* Smad2 Protein / metabolism* Transforming Growth Factor beta1 / metabolism*, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Transforming Growth Factor beta; 0/SMAD2 protein, human; 0/Smad2 Protein; 0/Smad2 protein, mouse; 0/Transforming Growth Factor beta1; EC 2.7.10.1/EGFR protein, human; EC 2.7.10.1/Receptor, Epidermal Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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