Document Detail


Effect of TGF-β on Gli2 expression in HL60 and U937 cell lines.
MedLine Citation:
PMID:  22395235     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Hedgehog (Hh) signaling pathway is involved in a variety of tumor-related diseases, including leukemia. The aim of this study was to determine whether there is an interaction between the Hh signaling pathway and transforming growth factor-β (TGF-β) in the HL60 and U937 cell lines. HL60 and U937 cells were treated with TGF-β, tumor necrosis factor-α (TNF-α) and specific inhibitor of Smad3 (SIS3). The expression of Gli2 was detected by real-time PCR and western blotting. The results showed that TGF-β alone did not affect the Gli2 expression in HL60 cells, while it significantly reduced the level of Gli2 expression in U937 cells. TGF-β+TNF-α reduced the expression of Gli2 in HL60 cells and U937 cells. The reduction in the level of Gli2 expression in U937 cells by TGF-β+TNF-α was greater than that caused by TGF-β alone. SIS3 inhibited the effect of TGF-β. Our results suggest that the effect on Gli2 expression in HL60 and U937 cells induced by TGF-β is Smad3-dependent and independent of Hh receptor signaling.
Authors:
Zhe Li; Deng Pan; Yan Li
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Publication Detail:
Type:  Journal Article     Date:  2012-03-02
Journal Detail:
Title:  Molecular medicine reports     Volume:  5     ISSN:  1791-3004     ISO Abbreviation:  Mol Med Rep     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-03-12     Completed Date:  2012-07-03     Revised Date:  2013-02-22    
Medline Journal Info:
Nlm Unique ID:  101475259     Medline TA:  Mol Med Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1245-50     Citation Subset:  IM    
Affiliation:
Department of Hematology, The First Affiliated Hospital, China Medical University, Shenyang, Liaoning 110001, PR China.
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MeSH Terms
Descriptor/Qualifier:
Gene Expression Regulation / drug effects*
HL-60 Cells
Hedgehog Proteins / metabolism
Humans
Kruppel-Like Transcription Factors / biosynthesis*
Nuclear Proteins / biosynthesis*
Signal Transduction / drug effects*
Smad3 Protein / metabolism*
Transforming Growth Factor beta / pharmacology*
Tumor Necrosis Factor-alpha / pharmacology*
U937 Cells
Chemical
Reg. No./Substance:
0/GLI2 protein, human; 0/Hedgehog Proteins; 0/Kruppel-Like Transcription Factors; 0/Nuclear Proteins; 0/Smad3 Protein; 0/Transforming Growth Factor beta; 0/Tumor Necrosis Factor-alpha

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