| Effect of TGF-β on Gli2 expression in HL60 and U937 cell lines. | |
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MedLine Citation:
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PMID: 22395235 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Hedgehog (Hh) signaling pathway is involved in a variety of tumor-related diseases, including leukemia. The aim of this study was to determine whether there is an interaction between the Hh signaling pathway and transforming growth factor-β (TGF-β) in the HL60 and U937 cell lines. HL60 and U937 cells were treated with TGF-β, tumor necrosis factor-α (TNF-α) and specific inhibitor of Smad3 (SIS3). The expression of Gli2 was detected by real-time PCR and western blotting. The results showed that TGF-β alone did not affect the Gli2 expression in HL60 cells, while it significantly reduced the level of Gli2 expression in U937 cells. TGF-β+TNF-α reduced the expression of Gli2 in HL60 cells and U937 cells. The reduction in the level of Gli2 expression in U937 cells by TGF-β+TNF-α was greater than that caused by TGF-β alone. SIS3 inhibited the effect of TGF-β. Our results suggest that the effect on Gli2 expression in HL60 and U937 cells induced by TGF-β is Smad3-dependent and independent of Hh receptor signaling. |
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Authors:
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Zhe Li; Deng Pan; Yan Li |
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Publication Detail:
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Type: Journal Article Date: 2012-03-02 |
Journal Detail:
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Title: Molecular medicine reports Volume: 5 ISSN: 1791-3004 ISO Abbreviation: Mol Med Rep Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-03-12 Completed Date: 2012-07-03 Revised Date: 2013-02-22 |
Medline Journal Info:
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Nlm Unique ID: 101475259 Medline TA: Mol Med Rep Country: Greece |
Other Details:
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Languages: eng Pagination: 1245-50 Citation Subset: IM |
Affiliation:
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Department of Hematology, The First Affiliated Hospital, China Medical University, Shenyang, Liaoning 110001, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Gene Expression Regulation
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drug effects* HL-60 Cells Hedgehog Proteins / metabolism Humans Kruppel-Like Transcription Factors / biosynthesis* Nuclear Proteins / biosynthesis* Signal Transduction / drug effects* Smad3 Protein / metabolism* Transforming Growth Factor beta / pharmacology* Tumor Necrosis Factor-alpha / pharmacology* U937 Cells |
| Chemical | |
Reg. No./Substance:
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0/GLI2 protein, human; 0/Hedgehog Proteins; 0/Kruppel-Like Transcription Factors; 0/Nuclear Proteins; 0/Smad3 Protein; 0/Transforming Growth Factor beta; 0/Tumor Necrosis Factor-alpha |
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