Document Detail

Effect of T-2 toxin on blood-brain barrier permeability monoamine oxidase activity and protein synthesis in rats.
MedLine Citation:
PMID:  9771558     Owner:  NLM     Status:  MEDLINE    
Systemic exposure to T-2 toxin disrupts brain biogenic monoamine metabolism. Although the mechanisms underlying these neurochemical perturbations are unclear, we have suggested that they are a reflection of increased blood-brain barrier (BBB) permeability, or altered protein synthesis that affects brain enzyme activities. Accordingly, BBB permeability, in vitro protein synthesis and in vitro monoamine oxidase (MAO) activity were examined in rats after either acute, or 7-day exposure to T-2. Membrane permeability was assessed from the recovery of systemically administered [14C]mannitol and [14C]dextran with [3H]water as the diffusible reference, either 2 hr post-intraperitoneal (i.p.) injections of 0, 0.2 and 1 mg T-2/kg body weight or following a 7-day exposure to diets containing 0 and 10 ppm T-2. Protein synthesis, determined by [14C]leucine incorporation, and MAO activity, determined by H2O2 production, were observed either 2 hr post-ip injection of 0 and 1 mg T-2/kg body weight or following a 7-day exposure to diets containing 0, 2.5 and 10 ppm T-2. Permeability increases were observed in all brain regions examined for mannitol, but not for dextran following T-2 i.p. The effect of dietary T-2 was more modest, affecting mannitol uptake in two brain regions, the cerebellum and pons plus medulla regions. Protein synthesis was significantly decreased by i.p. administration of T-2, while dietary treatment significantly reduced MAO enzyme activity. Collectively, the effect of T-2 toxin on BBB permeability, protein synthesis and MAO enzyme activity may account for the neurochemical imbalance observed in T-2 intoxication.
J Wang; D W Fitzpatrick; J R Wilson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  36     ISSN:  0278-6915     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1998-10-30     Completed Date:  1998-10-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  955-61     Citation Subset:  IM    
Department of Foods and Nutrition, University of Manitoba, Winnipeg, Canada.
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MeSH Terms
Biogenic Monoamines / metabolism
Blood-Brain Barrier / drug effects*
Carbon Radioisotopes
Cerebellum / drug effects*,  metabolism
Hydrogen Peroxide / analysis,  metabolism
Injections, Intraperitoneal
Mannitol / metabolism
Medulla Oblongata / drug effects,  metabolism
Monoamine Oxidase / metabolism*
Pons / drug effects*,  metabolism
Protein Biosynthesis*
Rats, Sprague-Dawley
T-2 Toxin / administration & dosage,  pharmacokinetics,  toxicity*
Reg. No./Substance:
0/Biogenic Monoamines; 0/Carbon Radioisotopes; 10028-17-8/Tritium; 21259-20-1/T-2 Toxin; 61-90-5/Leucine; 69-65-8/Mannitol; 7722-84-1/Hydrogen Peroxide; 9004-54-0/Dextrans; EC Oxidase

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