| Effect of Substrate Stiffness on Pulmonary Fibroblast Activation by TGF-β | |
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MedLine Citation:
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PMID: 22446029 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Peptide crosslinkers containing the sequence C-X-CG (X represents various adhesive peptides) were incorporated into PEG hydrogel networks with different mechanical properties. Pulmonary fibroblasts (PFs) exhibit increased adhesion to rigid hydrogels modified with X=RGDS, DGEA and IKVAV (0.5 and/or 5 mM) compared to a scrambled control (X=HRPNS). PFs exhibit increased adhesion to softer hydrogels when X=DGEA at low (0.5 mM) peptide concentration. PFs seeded onto hydrogels modified with X=RGDS produce alpha-smooth muscle actin (α-SMA), a myofibroblast marker, and form an extensive cytoskeleton with focal adhesions. Decreasing substrate stiffness (achieved through hydrolytic degradation) results in downregulation of α-SMA expression by PFs. Substrate stiffness increases the sensitivity of PFs to exogenously applied transforming growth factor beta (TGF-β1); PFs on the most rigid gels (E=900 kPa) express α-SMA when treated with low concentrations of TGF-β1 (1 ng/mL)while those on less rigid gels (E=20-60 kPa) do not. These results demonstrate the importance of both mechanical and chemical cues in studying pulmonary fibroblast activation and establish PEG hydrogels as a viable material for further studying IPF etiology. |
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Authors:
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Helena N Chia; Marina Vigen; Andrea M Kasko |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-3-20 |
Journal Detail:
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Title: Acta biomaterialia Volume: - ISSN: 1878-7568 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-3-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101233144 Medline TA: Acta Biomater Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier Ltd. |
Affiliation:
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Biomedical Engineering Interdepartmental Program, University of California, Los Angeles, CA 90095 United States. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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