Document Detail


The effect of stocking densities on reproductive performance in laboratory zebrafish (Danio rerio).
MedLine Citation:
PMID:  21854208     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Despite the growing popularity of the zebrafish model system, the optimal husbandry conditions for this animal are not well defined. The aim of this study was to examine the effect of stocking density on reproductive performance in zebrafish. In this study, undertaken by eight different zebrafish facilities, clutches of at least 200 wild-type zebrafish embryos from a single pairwise mating were produced at each participating institution and subsequently reared according to "in-house protocols" until they were 14 weeks old. Fish were then randomly assigned into treatment groups with balanced sex ratios and densities of 3, 6, or 12 fish/L. After a 1-month acclimation period, fish were spawned in pair crosses every 2 weeks for 3 months, for a total of six spawning dates. The number of viable and nonviable embryos produced in each clutch were counted at 1 day postfertilization. Although there was a great deal of variability in clutch size and percent spawning success among laboratories, there were no significant differences in average clutch size, spawning success, or percent viable among the treatment densities. These data suggest that using stocking densities as high as 12 fish/L does not have a negative impact on performance, when measured by reproductive performance.
Authors:
Daniel Castranova; Angela Lawton; Christian Lawrence; Diana P Baumann; Jason Best; Jordi Coscolla; Amy Doherty; Juan Ramos; Jenna Hakkesteeg; Chongmin Wang; Carole Wilson; James Malley; Brant M Weinstein
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-08-19
Journal Detail:
Title:  Zebrafish     Volume:  8     ISSN:  1557-8542     ISO Abbreviation:  Zebrafish     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-09-20     Completed Date:  2012-01-27     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  101225070     Medline TA:  Zebrafish     Country:  United States    
Other Details:
Languages:  eng     Pagination:  141-6     Citation Subset:  IM    
Affiliation:
Program in Genomics of Differentiation, NICHD, NIH, Bethesda, Maryland 20892, USA. castrand@mail.nih.gov
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MeSH Terms
Descriptor/Qualifier:
Animal Husbandry / methods*
Animals
Animals, Laboratory / physiology*
Clutch Size / physiology
Population Density
Random Allocation
Reproduction / physiology*
Zebrafish / physiology*
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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