Document Detail


Effect of St. John's wort on free radical production.
MedLine Citation:
PMID:  11441908     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
St. John's wort (Hypericum perforatum) is an herbal compound used in the treatment of burns, bruises, swelling, anxiety, and most recently, mild to moderate depression. The present study was designed to evaluate the antioxidant properties of St. John's wort in both cell-free and human vascular tissue. The experiment was performed initially in a cell-free system using Krebs buffer and a combination of xanthine/xanthine oxidase to initiate the production of the superoxide radical. Additionally, human placental vein was incubated in Krebs buffer without xanthine or xanthine oxidase to study the effects of St. John's wort on human tissue in vitro. Commercially available formulations of St. John's wort, standardized to either hypericin or hyperforin, were dissolved in an alkaline solution, and the following dilutions were made: 1:1, 1:2.5, 1:5, 1:7.5, 1:10, and 1:20. Lucigenin chemiluminescence was used to measure free radical production in both systems. A pro-oxidant effect was seen at the highest concentration, 1:1. Lower concentrations revealed antioxidant properties of the compound. All dilutions below 1:1 in both systems showed a dose-related inverse relationship of superoxide inhibition. The largest suppression was seen at the most dilute concentration, 1:20. The addition of 10(-3) M tiron inhibited the chemiluminescence signal, thereby confirming the production of superoxide. The results of this study suggest that St. John's wort inhibits free radical production in both cell-free and human vascular tissue.
Authors:
E J Hunt; C E Lester; E A Lester; R L Tackett
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Life sciences     Volume:  69     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-07-09     Completed Date:  2001-07-26     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  181-90     Citation Subset:  IM    
Affiliation:
University of Georgia College of Pharmacy, Department of Clinical and Administrative Sciences, Athens 30602, USA.
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MeSH Terms
Descriptor/Qualifier:
1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt / metabolism
Antioxidants / chemistry,  pharmacology*
Bicyclo Compounds
Cell-Free System
Dose-Response Relationship, Drug
Enzyme Inhibitors / chemistry,  pharmacology
Female
Free Radicals / metabolism
Humans
Hypericum* / chemistry
Indicators and Reagents / metabolism
Perylene / analogs & derivatives*,  chemistry,  pharmacology
Phloroglucinol / analogs & derivatives
Placenta / blood supply
Plant Extracts / pharmacology*
Plants, Medicinal*
Pregnancy
Superoxides / metabolism*
Terpenes / chemistry,  pharmacology
Veins / drug effects,  metabolism*
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Bicyclo Compounds; 0/Enzyme Inhibitors; 0/Free Radicals; 0/Indicators and Reagents; 0/Plant Extracts; 0/Terpenes; 108-73-6/Phloroglucinol; 11062-77-4/Superoxides; 11079-53-1/hyperforin; 149-45-1/1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt; 198-55-0/Perylene; 548-04-9/hypericin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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