Document Detail

Effect of resveratrol on gliotransmitter levels and p38 activities in cultured astrocytes.
MedLine Citation:
PMID:  20842424     Owner:  NLM     Status:  MEDLINE    
Accumulating evidence suggests that resveratrol may have beneficial effects against traumatic brain injury. However, its effect on the regulation of extracellular levels of gliotransmitter and on the activation of p38 MAPK in astrocytes is still unknown. We have examined whether resveratrol regulates extracellular levels of gliotransmitter as well as the activation of p38 MAPK in cultured astrocytes before and after stretch injury. The extracellular levels of glutamate, D-/L-serine and D-serine were apparently reduced by 100 μM resveratrol in control astrocyte cultures. The dramatic increase of glutamate and D-serine release induced by stretch injury was also clearly inhibited by resveratrol. Resveratrol mediates this response by reduction of release through inhibition of extracellular calcium influx and increment of gliotransmitter uptake through enhancement of amino acid transporter expressed in the membrane of astrocyte. In addition, resveratrol definitely reduced the activation of p38 MAPK in cultured astrocytes following stretch injury. AMPA receptor is involved in the activation of p38 following injury. Conversely, the levels of glutamine and glycine were not obviously affected by resveratrol before and after injury. Intracellular levels of glutamate and D-serine are not apparently changed by stretch injury. Collectively, our data suggest that resveratrol might play an important role in protection of the nervous system after injury by decreasing the extracellular levels of gliotransmitter and inhibiting activation of p38 MAPK following injury.
Hao Zhou; Qing Chen; Dan Li Kong; Jiang Guo; Qian Wang; Shou Yi Yu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-15
Journal Detail:
Title:  Neurochemical research     Volume:  36     ISSN:  1573-6903     ISO Abbreviation:  Neurochem. Res.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-28     Completed Date:  2011-04-18     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  17-26     Citation Subset:  IM    
Nanfang Hospital, Southern Medical University, Guangzhou, China.
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MeSH Terms
Amino Acids / metabolism
Astrocytes / cytology,  drug effects*,  metabolism*
Calcium / metabolism
Cells, Cultured
Chelating Agents / pharmacology
Culture Media / chemistry
Egtazic Acid / analogs & derivatives,  pharmacology
Enzyme Inhibitors / pharmacology*
Excitatory Amino Acid Transporter 2 / metabolism
Neurotransmitter Agents / metabolism*
Serine / metabolism
Stilbenes / pharmacology*
Stress, Mechanical
p38 Mitogen-Activated Protein Kinases / metabolism*
Reg. No./Substance:
0/Amino Acids; 0/Chelating Agents; 0/Culture Media; 0/Enzyme Inhibitors; 0/Excitatory Amino Acid Transporter 2; 0/Neurotransmitter Agents; 0/Stilbenes; 56-45-1/Serine; 67-42-5/Egtazic Acid; 7440-70-2/Calcium; EC Mitogen-Activated Protein Kinases; K22DDW77C0/1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; Q369O8926L/resveratrol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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