| Effect of ROCK inhibitor Y-27632 on normal and variant human embryonic stem cells (hESCs) in vitro: its benefits in hESC expansion. | |
| | |
MedLine Citation:
|
PMID: 20013076 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The Rho associated coiled coil protein kinase (ROCK) dependent signaling pathway plays an important role in numerous physiological functions such as cell proliferation, adhesion, migration and inflammation. Human embryonic stem cells (hESCs) undergo differentiation and poor survival after single cell dissociation in culture thus limiting their expansion for cell based therapies. We evaluated the role of the selective ROCK inhibitor Y-27632 on hESC colonies and disassociated single hESCs from two different hESC lines. Karyotypically normal hESCs (HES3) and variant hESCs (BG01V) were treated with Y-27632 at 5, 10 and 20 muM concentrations for 72 h and its effects on hESC self renewal, colony morphology, cell cycle and pluripotency were evaluated. Increased cell proliferation of both HES3 and BG01V were observed for all three concentrations compared to untreated controls following passaging of cell clusters or dissociated single cells and some of these increases were statistically significant. Cell cycle assay demonstrated normal cell cycle progression with no peaks evident of apoptosis. No morphological differentiation was evident following treatment with the highest concentration of Y-27632 (20 muM) and the stemness related genes continued to be highly expressed in both HES3 and BG01V cells compared to untreated controls. The results confirmed that Y-27632 is a useful agent that aids in the expansion of undifferentiated hESC numbers for downstream applications in regenerative medicine. |
| | |
Authors:
|
Kalamegam Gauthaman; Chui-Yee Fong; Ariff Bongso |
Related Documents
:
|
20654436 - Session 2: cell cycle and cell death. 16677426 - Metabolic cycle, cell cycle, and the finishing kick to start. 10717186 - Molecular biology of the cell cycle: potential for therapeutic applications in radiatio... 8753796 - Inhibition of the g2/m transition of the cell cycle by methyl-2,5-dihydroxycinnamate in... 18662576 - Detection of autophagy in cell death. 19383386 - Differentiation is coupled to changes in the cell cycle regulatory apparatus of human e... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-15 |
Journal Detail:
|
Title: Stem cell reviews Volume: 6 ISSN: 1558-6804 ISO Abbreviation: Stem Cell Rev Publication Date: 2010 Mar |
Date Detail:
|
Created Date: 2010-03-05 Completed Date: 2010-06-11 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101255952 Medline TA: Stem Cell Rev Country: United States |
Other Details:
|
Languages: eng Pagination: 86-95 Citation Subset: IM |
Affiliation:
|
Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Lower Kent Ridge Road, Singapore, 119074, Singapore. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amides
/
pharmacology* Animals Cell Culture Techniques Cell Cycle / drug effects Cell Differentiation / drug effects Cell Line Cell Proliferation / drug effects* Cell Shape / drug effects Dose-Response Relationship, Drug Embryonic Stem Cells / drug effects*, enzymology Humans Karyotyping Mice Pluripotent Stem Cells / drug effects*, enzymology Protein Kinase Inhibitors / pharmacology* Pyridines / pharmacology* Time Factors rho-Associated Kinases / antagonists & inhibitors*, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Amides; 0/Protein Kinase Inhibitors; 0/Pyridines; 138381-45-0/Y 27632; EC 2.7.11.1/rho-Associated Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Comparative analysis of antigen-targeting sequences used in DNA vaccines.
Next Document: A mimic of phosphorylated prolactin inhibits human breast cancer cell proliferation via upregulation...