Document Detail


Effect of Psidium cattleianum leaf extract on Streptococcus mutans viability, protein expression and acid production.
MedLine Citation:
PMID:  18367836     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plants naturally produce secondary metabolites that can be used as antimicrobials. The aim of this study was to assess the effects of Psidium cattleianum leaf extract on Streptococcus mutans. The extract (100%) was obtained by decoction of 100 g of leaves in 600 ml of deionized water. To assess killing, S. mutans biofilms were treated with water (negative control) or various extract dilutions [100, 50, 25% (v/v) in water] for 5 or 60 min. To evaluate the effect on protein expression, biofilms were exposed to water or 1.6% (v/v) extract for 120 min, proteins were extracted and submitted to 2-dimensional difference gel electrophoresis. Differentially expressed proteins were identified by mass spectrometry. The effect of 1.6% (v/v) extract on acid production was determined by pH measurements and compared to a water control. Viability was similar after 5 min of treatment with the 100% extract or 60 min with the 50% extract (about 0.03% survival). There were no differences in viability between the biofilms exposed to the 25 or 50% extract after 60 min of treatment (about 0.02% survival). Treatment with the 1.6% extract significantly changed protein expression. The abundance of 24 spots was decreased compared to water (p < 0.05). The extract significantly inhibited acid production (p < 0.05). It is concluded that P. cattleianum leaf extract kills S. mutans grown in biofilms when applied at high concentrations. At low concentrations it inhibits S. mutans acid production and reduces the expression of proteins involved in general metabolism, glycolysis and lactic acid production.
Authors:
F L Brighenti; S B I Luppens; A C B Delbem; D M Deng; M A Hoogenkamp; E Gaetti-Jardim; H L Dekker; W Crielaard; J M ten Cate
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-27
Journal Detail:
Title:  Caries research     Volume:  42     ISSN:  1421-976X     ISO Abbreviation:  Caries Res.     Publication Date:  2008  
Date Detail:
Created Date:  2008-04-30     Completed Date:  2008-06-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0103374     Medline TA:  Caries Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  148-54     Citation Subset:  D; IM    
Copyright Information:
(c) 2008 S. Karger AG, Basel
Affiliation:
Department of Cariology Endodontology Pedodontology, Academic Centre for Dentistry Amsterdam (ACTA), Universiteit van Amsterdam and Vrije Universiteit, Amsterdam, The Netherlands. ferbrighenti@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Bacterial Proteins / biosynthesis
Biofilms / drug effects
Colony Count, Microbial
Down-Regulation
Electrophoresis, Gel, Two-Dimensional
Hydrogen-Ion Concentration / drug effects
Lactic Acid / biosynthesis
Mass Spectrometry
Microbial Sensitivity Tests
Microbial Viability / drug effects
Phenols / pharmacology
Plant Extracts / pharmacology*
Plant Leaves
Psidium*
Statistics, Nonparametric
Streptococcus mutans / drug effects*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Phenols; 0/Plant Extracts; 50-21-5/Lactic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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