Document Detail

Effect of phytic acid on suicidal erythrocyte death.
MedLine Citation:
PMID:  20058927     Owner:  NLM     Status:  MEDLINE    
Phytic acid, an anticarcinogenic food component, stimulates apoptosis of tumor cells. Similar to apoptosis, human erythrocytes may undergo suicidal death or eryptosis, characterized by cell membrane scrambling and cell shrinkage. Triggers of eryptosis include energy depletion. Phytate intake could cause anemia, an effect attributed to iron complexation. The present experiments explored whether phytic acid influences eryptosis. Supernatant hemoglobin concentration was determined to reveal hemolysis, annexin V-binding in FACS analysis was utilized to identify erythrocytes with scrambled cell membrane, forward scatter in FACS analysis was taken as a measure of cell volume, and a luciferin-luciferase assay was employed to determine erythrocyte ATP content. As a result, phytic acid (>or=1 mM) did not lead to significant hemolysis, but significantly increased the percentage of annexin V-binding erythrocytes, significantly decreased forward scatter, and significantly decreased cellular ATP content. In conclusion, phytic acid stimulates suicidal human erythrocyte death, an effect paralleling its proapoptotic effect on nucleated cells.
Matthias Eberhard; Michael F?ller; Florian Lang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  58     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-03     Completed Date:  2010-04-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2028-33     Citation Subset:  IM    
Department of Physiology, Eberhard-Karls-University of Tubingen, Gmelinstrasse 5, D-72076 Tuebingen, Germany.
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MeSH Terms
Adenosine Triphosphate / metabolism
Cell Death / drug effects
Erythrocytes / cytology*,  drug effects*,  metabolism
Hemoglobins / metabolism
Hemolysis / drug effects*
Phytic Acid / pharmacology*
Reg. No./Substance:
0/Hemoglobins; 56-65-5/Adenosine Triphosphate; 83-86-3/Phytic Acid

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