| The effect of PTEN on serotonin synthesis and secretion from the carcinoid cell line BON. | |
MedLine Citation:
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PMID: 21508359 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Carcinoid tumors are associated with the carcinoid syndrome, a set of symptoms resulting from the peptide and amine products, including serotonin, secreted from the cancer cells. The purpose of this study was to investigate the relationship between the phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt) inhibitor PTEN (phosphatase and tensin homolog deleted on chromosome ten) and serotonin synthesis and secretion in the carcinoid cancer cell line BON. MATERIALS AND METHODS: PTEN was inhibited by pharmacological and molecular approaches, and the resultant secretion of serotonin and expression of tryptophan hydroxylase 1 (TPH1), the rate-limiting enzyme in serotonin synthesis, was assessed. RESULTS: Inhibition of PTEN in vitro, with concomitant increased Akt signaling, resulted in decreased secretion of serotonin, as well as decreased serotonin synthesis, as confirmed by reduced expression of TPH1. Inhibition of PTEN in BON cells in an animal model resulted in decreased serum serotonin. CONCLUSION: By inhibiting signaling through Akt, PTEN indirectly promotes serotonin synthesis and secretion. |
Authors:
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Scott R Silva; Yekaterina Y Zaytseva; Lindsey N Jackson; Eun Y Lee; Heidi L Weiss; Kanika A Bowen; Courtney M Townsend; B Mark Evers |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Anticancer research Volume: 31 ISSN: 1791-7530 ISO Abbreviation: Anticancer Res. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-04-21 Completed Date: 2011-07-26 Revised Date: 2013-06-30 |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
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Languages: eng Pagination: 1153-60 Citation Subset: IM |
Affiliation:
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Markey Cancer Center, University of Kentucky, Lexington, KY 40536-0093, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Carcinoid Tumor / drug therapy*, metabolism, pathology Enzyme-Linked Immunosorbent Assay Female Immunoenzyme Techniques Liver Neoplasms / drug therapy*, metabolism, secondary Mice Mice, Nude PTEN Phosphohydrolase / antagonists & inhibitors, pharmacology* Phosphatidylinositol 3-Kinases / antagonists & inhibitors Proto-Oncogene Proteins c-akt / antagonists & inhibitors RNA, Small Interfering / genetics Serotonin / metabolism* Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA104748/CA/NCI NIH HHS; R01 CA104748-05/CA/NCI NIH HHS; R01 DK048498-17/DK/NIDDK NIH HHS; R01 DK48489/DK/NIDDK NIH HHS; R37 AG010885-20/AG/NIA NIH HHS; R37 AG10885/AG/NIA NIH HHS; T32 DK007639-16/DK/NIDDK NIH HHS; T32DK07639/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Small Interfering; 50-67-9/Serotonin; EC 2.7.1.-/Phosphatidylinositol 3-Kinases; EC 2.7.11.1/Proto-Oncogene Proteins c-akt; EC 3.1.3.48/PTEN protein, human; EC 3.1.3.67/PTEN Phosphohydrolase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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