Document Detail


The Effect of Organic Modifiers on Electrospray Ionization Charge-State Distribution and Desorption Efficiency for Oligonucleotides.
MedLine Citation:
PMID:  23325666     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The chemical composition of the solution has a critical impact on the electrospray desorption efficiency of oligonucleotides. Several physiochemical properties of various organic modifiers were investigated with respect to their role in the desorption process of oligonucleotides. The Henry's Law Constant, which reflects the volatility of alkylamines, was found to have a prominent effect on both the electrospray charge state distribution and desorption efficiency of oligonucleotides. Alkylamines with higher [Formula: see text] values such as hexylamine, piperidine, and imidazole reduced the charge state distribution by forming complexes with the oligonucleotide and dissociating from it in the gas phase, while alkylamines with extremely low [Formula: see text] values reduced the electrospray charge state distribution by facilitating ion emission at an earlier stage of the electrospray desorption process. Ion-pairing agents with moderate [Formula: see text] values do not alter the electrospray charge state distribution of oligonucleotides and their ability to enhance oligonucleotide ionization followed the order of decreasing [Formula: see text] values. The Henry's Law Constant also correlated to the impact of the acidic modifiers on oligonucleotide ionization efficiency. Ionization enhancement effects were observed with hexafluoroisopropanol, and this effect was attributed to its low [Formula: see text] and moderate acidity. The comprehensive effects of both alkylamine and hexafluoroisoproapnol on the electrospray ionization desorption of oligonucleotides were also evaluated, and acid-base equilibrium was found to play a critical role in determining these effects.
Authors:
Buyun Chen; Sadie F Mason; Michael G Bartlett
Related Documents :
3191526 - Influence of ca2+ on the isolation from rat brain mitochondria of a fraction enriched o...
2077756 - A study on the regulation of translocation of glucose transporters during hepatocarcino...
3867666 - Study on membrane fragments released from the sublingual glands of the mouse during sec...
3368026 - Cerebral metabolism of plasma [14c]palmitate in awake, adult rat: subcellular localizat...
19573986 - A comparative study on the membrane based palm oil mill effluent (pome) treatment plant.
23615196 - The bacterial magnetosome: a unique prokaryotic organelle.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-17
Journal Detail:
Title:  Journal of the American Society for Mass Spectrometry     Volume:  -     ISSN:  1879-1123     ISO Abbreviation:  J. Am. Soc. Mass Spectrom.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9010412     Medline TA:  J Am Soc Mass Spectrom     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Pharmaceutical and Biomedical Science, College of Pharmacy, The University of Georgia, Athens, GA, 30602-2352, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Gene expression profiling in the cerebral cortex of patients with cirrhosis with and without hepatic...
Next Document:  De Novo Biosynthesis of Sexual Pheromone in the Labial Gland of Bumblebee Males.