Document Detail

Effect of N8-acetylspermidine deacetylase inhibition on the growth of L1210 cells.
MedLine Citation:
PMID:  11230796     Owner:  NLM     Status:  MEDLINE    
A selective inhibitor of N8-acetylspermidine deacetylase has been employed to study the role of N8-acetylspermidine deacetylation in the regulation of L1210 cell growth. This inhibitor, 7-[N-(3-aminopropyl) amino] heptan-2-one (APAH), was found to stimulate the growth of L1210 cells at concentrations between 10 microM and 0.5 mM. Maximum stimulation was seen at 100 microM, resulting in significantly increased rates of cell division and maximum cell density. N8-Acetylspermidine levels in L1210 cells were shown to increase significantly after the APAH treatment as would be expected for deacetylase inhibition. The effects of deacetylase inhibition were mimicked by addition of N8-acetylspermidine to the culture medium at concentrations greater than 1 mM as indicated by a subsequent increase in rate of cell growth and maximum cell density. The magnitudes of the increases in growth observed were not large, but this might be expected in cells that are already in a rapid growth phase. Other exogenously added polyamines including N1-acetylspermidine, spermidine, putrescine, and spermine did not stimulate cell growth. These data suggest that stimulation of cell growth occurs as a consequence of N8-acetylspermidine accumulation and N8-acetylspermidine deacetylase inhibition.
Z Wang; D Fries; J Blankenship
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Biochemical pharmacology     Volume:  57     ISSN:  0006-2952     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  1999 May 
Date Detail:
Created Date:  2001-03-20     Completed Date:  2001-04-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1095-103     Citation Subset:  IM    
Departments of Physiology and Pharmacology, School of Pharmacy, University of the Pacific, Stockton, CA 95211, USA.
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MeSH Terms
Amidohydrolases / antagonists & inhibitors,  physiology*
Cell Division / drug effects,  physiology*
Chromatography, High Pressure Liquid
Diamines / pharmacology*
Drug Interactions
Enzyme Inhibitors / pharmacology*
Leukemia L1210
Polyamines / metabolism
Putrescine / pharmacology
Spermidine / analogs & derivatives*,  pharmacology*
Spermine / pharmacology
Reg. No./Substance:
0/Diamines; 0/Enzyme Inhibitors; 0/Polyamines; 110-60-1/Putrescine; 122269-09-4/7-(N-(3-aminopropyl)amino)heptan-2-one; 124-20-9/Spermidine; 13431-24-8/N(8)-acetylspermidine; 14278-49-0/N(1)-acetylspermidine; 71-44-3/Spermine; EC 3.5.-/Amidohydrolases; EC deacetylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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