Document Detail

Effect of N-nitroso-N-methylurethane on gas exchange, lung compliance, and surfactant function of rabbits.
MedLine Citation:
PMID:  8708173     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: To define the effect of N-nitroso-N-methyl-urethane (NNNMU) on pulmonary gas exchange, compliance and the biochemical and functional properties of the lung surfactant system. DESIGN: Four days after inducing lung injury, gas exchange and pulmonary compliance were studied and a bronchoalveolar lavage was taken. SETTING: Experimental laboratory of a university department of medicine, division of pulmonary and critical care medicine. ANIMALS: Ten rabbits after they had received an injection of NNNMU and five control animals. INTERVENTIONS: Controlled mechanical ventilation and bronchoalveolar lavage. MEASUREMENTS AND RESULTS: Measurements of gas exchange (using the multiple inert gas elimination technique), hemodynamics and pulmonary compliance were performed during ventilatory and hemodynamic steady state. A bronchoalveolar lavage (BAL) was taken after sacrificing the animal. BAL samples were processed for cell count and biochemical and functional surfactant analysis. Animals injected with NNNMU developed mild, but significant reduction in PaO2, while maintaining eucapnia during spontaneous air breathing. V/Q distributions and arterial blood gases were similar in all animals when ventilated mechanically with a fixed tidal volume. Compliance of the lung and phospholipid levels in lavage of NNNMU animals was significantly lower than in control animals (CON). Function of surfactant recovered from animals receiving NNNMU was decreased significantly where compared to CON. Thus, NNNMU resulted in a lowered lavage surfactant phospholipid content, impaired surfactant function, decreased compliance and hypoxemia during spontaneous ventilation. However, gas exchange was similar to that of control animals during mechanical ventilation. CONCLUSION: We conclude that NNNMU-induced gas exchange abnormalities present after 4 days are mild and are reversed by fixed volume mechanical ventilation despite marked alteration in surfactant function and lung compliance. These observations further define properties of a lung injury model that is of value in the study of surfactant replacement.
D Pappert; N Gilliard; G Heldt; T A Merritt; P D Wagner; R G Spragg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Intensive care medicine     Volume:  22     ISSN:  0342-4642     ISO Abbreviation:  Intensive Care Med     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1996-09-09     Completed Date:  1996-09-09     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7704851     Medline TA:  Intensive Care Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  345-52     Citation Subset:  IM    
Klinik für Anaesthesiologie, und operative Intensivmedizin, WE01 Universitätsklinikum, Rudolf Virchow, Freie Universität Berlin, Germany.
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MeSH Terms
Blood Gas Analysis
Bronchoalveolar Lavage
Hemodynamics / drug effects
Lung Compliance / drug effects*
Nitrosomethylurethane / pharmacology
Pulmonary Gas Exchange / drug effects*
Pulmonary Surfactants / drug effects*,  physiology
Respiratory Distress Syndrome, Adult / physiopathology*
Grant Support
Reg. No./Substance:
0/Pulmonary Surfactants; 615-53-2/Nitrosomethylurethane

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