| Effect of N-acetyl cysteine on orthodontic primers cytotoxicity. | |
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MedLine Citation:
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PMID: 21081246 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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OBJECTIVES: The aims of this study were to evaluate the cytotoxicity of four orthodontic primers, including two hydrophilic and two hydrophobic materials, and to investigate the role of the reactive oxygen species (ROS) in induced cell damage. Moreover, the effects of the anti-oxidant N-acetyl cysteine (NAC) on primers toxicity was analyzed. METHODS: Human gingival fibroblasts (HGF) were exposed to different concentrations of primers (0-0.25 mg/ml) in the presence or absence of NAC, and the cytotoxicity was assessed by the MTT assay, while cell death was quantified by flow cytometry after propidium iodide staining. The increase in the induced ROS levels was detected by flow cytometry measuring the fluorescence of the oxidation-sensitive dye 2',7'-dichlorofluorescein diacetate (DCFH-DA). RESULTS: All materials decreased cell viability in a dose-related manner after a 24 h exposure period. Cytotoxicity of orthodontic primers based on concentrations which caused a 50% decrease in cell viability (TC₅₀) in HGF was ranked as follows (median values): Eagle Fluorsure (0.078 mg/ml)>Transbond XT (0.081 mg/ml)>Transbond MIP (0.128 mg/ml)>Ortho solo (0.130 mg/ml). Moreover, in HGF cells, all materials induced a dose-dependent increase in ROS levels compared to untreated cells. Incubation of HGF with NAC significantly reduced ROS production and decreased the cell damage and cytotoxicity caused by all materials tested (p<0.001). SIGNIFICANCE: Our results suggested that hydrophilic primers were less cytotoxic than hydrophobic materials. Moreover, we demonstrated a major role of ROS in the induction of cell death since the antioxidant N-acetyl cysteine was able to prevent cell damage induced by all materials tested. |
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Authors:
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Vincenzo D'Antò; Gianrico Spagnuolo; Helmut Schweikl; Sandro Rengo; Luigi Ambrosio; Roberto Martina; Rosa Valletta |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Dental materials : official publication of the Academy of Dental Materials Volume: 27 ISSN: 1879-0097 ISO Abbreviation: Dent Mater Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8508040 Medline TA: Dent Mater Country: England |
Other Details:
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Languages: eng Pagination: 180-6 Citation Subset: D |
Copyright Information:
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Copyright © 2010 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Oral and Maxillofacial Sciences, University of Naples Federico II, via S. Pansini 5, 80128 Napoli, Italy. vincenzo.danto@unina.it |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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