Document Detail

Effect of N-3 polyunsaturated fatty acid supplemented diet on neutrophil-mediated ileal permeability and neutrophil function in the rat.
MedLine Citation:
PMID:  8586775     Owner:  NLM     Status:  MEDLINE    
RATIONALE AND OBJECTIVE: Fish oil, rich in n-3 polyunsaturated fatty acids, can alter leukotriene production and hence neutrophil function, factors which may be important in the inflammation of Crohn's disease (CD). Therefore we studied the effect of dietary PUFA on neutrophil mediated ileal inflammation and neutrophil function in the rat. METHODS: Animals were ad libitum-fed pellet diets containing 9.5% fish oil (menhaden oil, rich in n-3 PUFA) with 0.5% safflower oil, 10% safflower oil (rich in n-6 PUFA) or standard chow (6% fat) for 50 days prior to the study. Weight and circulating leukocyte and total neutrophil counts were identical in all three groups. Neutrophil mediated ileal inflammation induced by formyl-methionyl-leucyl-phenylalanine (fMLP) perfusion was evaluated by measuring macromolecular uptake of radiolabelled dextran (MW 70,000) and changes in mucosal neutrophil infiltration. RESULTS: The fish oil diet group showed no difference in fMLP-induced permeability changes relative to the Chow Control group. However, the Safflower Oil supplemented diet group had a reduced permeability response (p < 0.01). Mirroring the permeability changes, there was diminished mucosal neutrophil infiltration in the Safflower Oil group following ileal perfusion with fMLP (< .005). Chemotaxis and chemiluminescence, two important neutrophil functions, were also significantly suppressed in the Safflower Oil animals (p < 0.05). CONCLUSIONS: The failure of a n-3 PUFA enriched diet to diminish the ileal inflammatory response to a bacterial peptide and suppress neutrophil function in the rat suggests such therapy would not be expected to be highly successful in CD. However, it requires confirmation in man, especially under the more complicated inflammatory conditions found in CD. On the other hand, the decreased neutrophil mediated responses with a high linoleic acid (n-6 PUFA) diet warrant further investigation.
A Chawla; P I Karl; S E Fisher
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the American College of Nutrition     Volume:  14     ISSN:  0731-5724     ISO Abbreviation:  J Am Coll Nutr     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1996-03-28     Completed Date:  1996-03-28     Revised Date:  2008-06-23    
Medline Journal Info:
Nlm Unique ID:  8215879     Medline TA:  J Am Coll Nutr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  258-63     Citation Subset:  IM    
Department of Pediatrics, North Shore University Hospital-Cornell University Medical College, Manhasset, New York, USA.
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MeSH Terms
Chemiluminescent Measurements
Chemotaxis, Leukocyte
Crohn Disease / metabolism,  pathology,  physiopathology
Dextrans / pharmacokinetics
Disease Models, Animal
Fatty Acids, Omega-3 / administration & dosage,  pharmacology*
Food, Fortified
Ileum / cytology,  metabolism*,  physiology
Leukotrienes / metabolism
Linoleic Acid
Linoleic Acids / pharmacology
N-Formylmethionine Leucyl-Phenylalanine / pharmacology
Neutrophils / drug effects,  physiology*
Rats, Sprague-Dawley
Reg. No./Substance:
0/Fatty Acids, Omega-3; 0/Leukotrienes; 0/Linoleic Acids; 2197-37-7/Linoleic Acid; 59880-97-6/N-Formylmethionine Leucyl-Phenylalanine; 9004-54-0/Dextrans

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