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Effect of Modulation of hnRNP L Levels on the Decay of bcl-2 mRNA in MCF-7 Cells.
MedLine Citation:
PMID:  20221275     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
It has been shown that CA repeats in the 3'-untranslated region (UTR) of bcl-2 mRNA contribute the constitutive decay of bcl-2 mRNA and that hnRNP L (heterogenous nuclear ribonucleoprotein L) interacts with CA repeats in the 3'-UTR of bcl-2 mRNA, both in vitro and in vivo. The aim of this study was to determine whether the alteration of hnRNP L affects the stability of bcl-2 mRNA in vivo. Human breast carcinoma MCF-7 cells were transfected with hnRNP L-specific shRNA or hnRNP L-expressing vector to decrease or increase hnRNP L levels, respectively, followed by an actinomycin D chase. An RT-PCR analysis showed that the rate of degradation of endogenous bcl-2 mRNA was not affected by the decrease or increase in the hnRNP L levels. Furthermore, during apoptosis or autophagy, in which bcl-2 expression has been reported to decrease, no difference in the degradation of bcl-2 mRNA was observed between control and hnRNP L-knock down MCF-7 Cells. On the other hand, the levels of AUF-1 and nucleolin, transacting factors for ARE in the 3'UTR of bcl-2 mRNA, were not significantly affected by the decrease in hnRNP L, suggesting that a disturbance in the quantitative balance between these transacting factors is not likely to interfere with the effect of hnRNP L. Collectively, the findings indicate that the decay of bcl-2 mRNA does not appear to be directly controlled by hnRNP L in vivo.
Authors:
Mi-Hyun Lim; Dong-Hyoung Lee; Seung Eun Jung; Dong-Ye Youn; Chan Sun Park; Jeong-Hwa Lee
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Publication Detail:
Type:  Journal Article     Date:  2010-02-28
Journal Detail:
Title:  The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology     Volume:  14     ISSN:  1226-4512     ISO Abbreviation:  Korean J. Physiol. Pharmacol.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-03-11     Completed Date:  2011-07-14     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  9709505     Medline TA:  Korean J Physiol Pharmacol     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  15-20     Citation Subset:  -    
Affiliation:
Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea.
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