| Effect of Mobile Phase Additives on the Resolution of Four Bioactive Compounds by RP-HPLC. | |
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MedLine Citation:
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PMID: 20559512 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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The use of mobile phase additives enhances the separation and resolution of the bioactive compounds on the C(18) column. Chlorogenic acid, caffeic acid, rutin, and scoparone from Herba Artemisiae Scopariae were investigated as the target compounds. Acetic acid, triethylamine, inorganic salts, and several ionic liquids were added as mobile phase additives into methanol/water (40:60, v/v). The result revealed that a mobile phase with 0.01 mol/L of ionic liquid [BMIM][BF(4)] enabled the optimum separation of the four target compounds. |
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Authors:
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Shengnan Li; Minglei Tian; Kyung Ho Row |
Publication Detail:
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Type: Journal Article Date: 2010-05-25 |
Journal Detail:
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Title: International journal of molecular sciences Volume: 11 ISSN: 1422-0067 ISO Abbreviation: Int J Mol Sci Publication Date: 2010 |
Date Detail:
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Created Date: 2010-06-18 Completed Date: 2011-07-14 Revised Date: 2013-05-29 |
Medline Journal Info:
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Nlm Unique ID: 101092791 Medline TA: Int J Mol Sci Country: Switzerland |
Other Details:
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Languages: eng Pagination: 2229-40 Citation Subset: - |
Affiliation:
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Department of Chemical Engineering, Inha University, Incheon 402-751, Korea; E-Mails: lishengnank@hotmail.com (S.L.); feitiandezhu@hotmail.com (M.T.). |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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