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Effect of Microfluidization on <i>in Vitro</i> Micellization and Intestinal Cell Uptake of Lutein from <i>Chlorella vulgaris</i>
MedLine Citation:
PMID:  21776960     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
<i>Chlorella</i> is a nutrient-rich microalga that contains protein, lipid, minerals, vitamins, and high levels of lutein. This study evaluated the bioavailability of lutein from <i>Chlorella vulgaris</i> using a coupled <i>in vitro</i> digestion and human intestinal Caco-2 cell model. Lutein bioavailability was low and approximately 75% of total <i>C. vulgaris</i> lutein was not micellized during the digestion process, but remained in the insoluble digestate. Microfluidization improved lutein micellization efficiency during <i>C. vulgaris</i> digestion. <i>C. vulgaris</i> was microfluidized at a pressure exceeding 10,000 psi and the cell surface disruption was visualized by scanning electron microscopy. The mean <i>C. vulgaris</i> particle size was reduced from 3.56 to 0.35 μm with the microfluidization treatment. <i>C. vulgaris</i> microfluidization at 20,000 psi was three times more efficient for aqueous lutein micelles production compared with untreated <i>C. vulgaris</i>, and the final lutein content accumulated by intestinal Caco-2 cells was also higher with microfluidization. <i>C. vulgaris</i> lutein stability was not affected by microfluidization. These results indicate that microfluidization may be useful for improving lutein bioavailability from <i>C. vulgaris</i> during food processing.
Authors:
Kwang Hyun Cha; Joo Young Lee; Dae-Geun Song; Sang Min Kim; Dong-Un Lee; Jin-Young Jeon; Cheol-Ho Pan
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-20
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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