| The effect of the major components of Fructus Cnidii on osteoblasts in vitro. | |
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MedLine Citation:
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PMID: 20633513 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In traditional Chinese medicine, the cause of weak bones or bone loss is generally regarded as a result of kidney deficiency. Fructus Cnidii (FC), which is also known as She-Chuang-Zi, is a traditional herb that has been claimed to have kidney warming effects that invigorate Yang. In this study, we tried to determine the bone production-inducing effect of FC on osteoblastic cells in vitro using osthole, the main component of FC. Osteoblasts were isolated from neonatal Sprague-Dawley rat calvaria using the tissue piece culture method and treated with various concentrations of osthole ranging from 2.5 to 640 microg/mL, together with a blank control. Cell proliferation, alkaline phosphatase (ALP) activity, and bone nodules were measured. The cells were examined by hematoxylin-eosin staining, the Gomori Calcium-Cobalt method and immunofluorescent staining. The 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (or MTT) assay, ALP assay, and bone nodule results indicated significantly enhanced osteoblastic proliferation and differentiation at concentrations of osthole ranging from 40 to 320 microg/mL. Concentrations lower than 40 microg/ mL seemed less effective, and cytotoxicity to osteoblasts was observed at concentrations higher than 320 microg/mL. These results indicate that osthole is effective at inducing osteoblastic bone formation through the up-regulation of ALP activity. FC is a Chinese herb used to treat lumbar pain in clinical practice. Further studies concerning the effects and mechanism of osthole on osteoporotic patients and animals should be performed, as these studies may lead to the development of a drug treatment for osteoporosis in the future. |
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Authors:
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Wenping Zhang; Dongming Ma; Qiduo Zhao; Torao Ishida |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of acupuncture and meridian studies Volume: 3 ISSN: 2005-2901 ISO Abbreviation: J Acupunct Meridian Stud Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-07-16 Completed Date: 2010-10-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101490763 Medline TA: J Acupunct Meridian Stud Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 32-7 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Korean Pharmacopuncture Institute. Published by .. All rights reserved. |
Affiliation:
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Department of Acupuncture, Suzuka University of Medical Science, Mie, Japan. zwp2000@suzuka-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation / drug effects Cell Proliferation / drug effects Cells, Cultured Cornus / chemistry* Drugs, Chinese Herbal / pharmacology*, toxicity Osteoblasts / cytology, drug effects* Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Drugs, Chinese Herbal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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