Document Detail


The effect of lyophilization on graft acceptance in experimental xenotransplantation using porcine cornea.
MedLine Citation:
PMID:  19821814     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The immunogenicity of lyophilized porcine cornea is unknown. The purpose of this study was to examine the possibility of using lyophilized porcine cornea as a substrate for ocular surface reconstruction. A porcine cornea stromal button was freeze-dried and vacuum-packed. Lyophilized and fresh porcine corneas were examined histologically, and then implanted into intrastromal pockets in live rat corneas. Cytokine concentrations in plasma and protein extracts from the corneal buttons of rats were measured using the fluorokine multianalyte profiling assay, and histologic examination was performed. Immunoreactivity to the alpha-gal epitope was not found in lyophilized porcine corneas, whereas it was found in several keratocytes in fresh porcine corneas. The median survival time of rat corneas receiving lyophilized porcine transplants was 28.0 days, significantly longer than the 14.0-day survival of rat corneas that received fresh porcine transplants (P < 0.05). CD45RO(+) and CD68(+) cells were observed in rejected corneas, and interleukin-2 and interferon-gamma were elevated in rat plasma and corneal tissue. The lyophilized porcine corneal stroma, which is devoid of alpha-gal epitope, is less antigenic, and may be a useful biomaterial for ocular surface reconstruction and corneal collagen supplementation.
Authors:
Jeong-Kyu Lee; Yang-Hwan Ryu; Jae-Il Ahn; Mi-Kyung Kim; Tae-Soo Lee; Jae-Chan Kim
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Publication Detail:
Type:  Journal Article     Date:  2009-10-11
Journal Detail:
Title:  Artificial organs     Volume:  34     ISSN:  1525-1594     ISO Abbreviation:  Artif Organs     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-04-27     Completed Date:  2010-08-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7802778     Medline TA:  Artif Organs     Country:  United States    
Other Details:
Languages:  eng     Pagination:  37-45     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, Chung-Ang University Hospital, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cornea / immunology*
Corneal Transplantation*
Cytokines / blood
Freeze Drying
Graft Rejection / immunology*,  prevention & control
Immunohistochemistry
Rats
Rats, Sprague-Dawley
Swine
Transplantation, Heterologous
Chemical
Reg. No./Substance:
0/Cytokines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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