| Effect of Leukemia Inhibitory Factor on the Myelinogenic Ability of Schwann-Like Cells Induced from Human Adipose-Derived Stem Cells. | |
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MedLine Citation:
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PMID: 23212292 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The Schwann cells (SCs) may be obtain from nerve biopsies for autologous transplantation. However, it is difficult to obtain sufficient amount of SCs for clinical applications. Human adipose-derived stem cells (ADSCs) can be induced to differentiate into Schwann-like cells (S-like cells) and used for autologous transplantation. However, effect of leukemia inhibitory factor (LIF) on the myelinogenic ability of SC-like cells induced from human ADSC is not investigated yet. The aim of this study was to evaluate of the effect of exogenous LIF on myelinogenic potential of differentiated cells in vitro. ADSCs were harvested from human fat tissue and characterized using flow cytometry. Human ADSCs were treated for sphere formation and LIF was added to terminal differentiation medium. GFAP/S100β and MBP markers were used to confirm differentiation of human ADSCs, and myelinogenic ability of SC-like cells, respectively, using both immunostaining and real-time RT-PCR analysis. The analysis for GFAP(+)/S100β(+) revealed that LIF can increase both differentiated cells rates and the percentage of myelinating SC-like cells (p < 0.05). Our data showed that SC-like cells induced from human ADSCs were able to generate myelin when exposed to LIF and these cells could be a potential source for the treatment of peripheral and central axonal injuries. |
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Authors:
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Shahnaz Razavi; Mohammad Mardani; Mohammad Kazemi; Ebrahim Esfandiari; Manizheh Narimani; Abolghasem Esmaeili; Nafiseh Ahmadi |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-12-5 |
Journal Detail:
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Title: Cellular and molecular neurobiology Volume: - ISSN: 1573-6830 ISO Abbreviation: Cell. Mol. Neurobiol. Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-12-5 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8200709 Medline TA: Cell Mol Neurobiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, 81744-176, Isfahan, Iran, razavi@med.mui.ac.ir. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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