| Effect of L-carnitine on the synthesis of nitric oxide in RAW 264·7 murine macrophage cell line. | |
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MedLine Citation:
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PMID: 22012571 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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L-Carnitine (β-hydroxy-γ-trimethyl aminobutyric acid) plays a critical role in inflammatory diseases by modulating inflammatory cell functions. Inducible nitric oxide synthase (iNOS), a proinflammatory enzyme responsible for the generation of nitric oxide (NO), has been implicated in the pathogenesis of inflammatory diseases. Mechanism of action of L-carnitine on inflammation via iNOS and nuclear factor κB (NF-κB) is unclear. In this study, we aimed to investigate the effect of L-carnitine on nitric oxide synthesis in lipopolysaccharide (LPS)-stimulated RAW 264·7 macrophage cells. For this purpose, cells were pretreated with various concentrations of L-carnitine and subsequently incubated with LPS (1 µg·ml(-1) ). NO levels, iNOS protein expression, and NF-κB activity were determined using colorimetric detection, Western blotting and transfection assays. Our results showed that treatment with L-carnitine suppressed nitric oxide production, iNOS protein expression and NF-κB activity. We demonstrated that inhibitory effect of L-carnitine on iNOS protein expression is at transcriptional level. This study may contribute to understanding the anti-inflammatory effect of L-carnitine. Copyright © 2011 John Wiley & Sons, Ltd. |
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Authors:
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A Koc; T Ozkan; A Z Karabay; A Sunguroglu; F Aktan |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-19 |
Journal Detail:
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Title: Cell biochemistry and function Volume: - ISSN: 1099-0844 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8305874 Medline TA: Cell Biochem Funct Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 John Wiley & Sons, Ltd. |
Affiliation:
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Department of Biochemistry, Ankara University Faculty of Pharmacy, Ankara, Turkey. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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