| Effect of L-arginine on renal tubular bicarbonate reabsorption by the rat kidney. | |
| | |
MedLine Citation:
|
PMID: 2761488 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
This study was designed to characterize the effect of L-arginine, a dibasic amino acid, on bicarbonate reabsorption (RHCO3) by the superficial proximal convoluted tubule using the technique of microperfusion in situ and on overall kidney RHCO3 using clearance techniques. Luminal perfusion of proximal tubules with L-arginine (5, 10, and 20 mM) resulted in a concentration-dependent inhibition of RHCO3. Capillary perfusion with L-arginine (10 mM) had no significant effect indicating that this amino acid must gain access into the cell from the luminal side to inhibit RHCO3. The inhibitory effect of L-arginine on RHCO3 was stereospecific since luminal perfusion with D-arginine (10 mM) had no significant effect on RHCO3. The lowest concentration of luminal L-arginine (5 mM) which produced a significant inhibitory effect on RHCO3 (about 13% inhibition) was comparable to that measured in the blood of rats infused with this amino acid (6.4 +/- 0.7 mM). Systemic infusion of L-arginine resulted in metabolic acidosis and only a slight increase in HCO3 excretion. To investigate whether the lack of overt bicarbonaturia was due to the reduced filtered load of HCO3 or to a stimulatory effect of acidemia on distal HCO3 reabsorption, blood bicarbonate was elevated by the infusion of NaHCO3. Under those conditions overall kidney absolute RHCO3, examined as a function of filtered load, was maximal in rats infused with L-arginine, thereby suggesting that when proximal HCO3 transport is inhibited, RHCO3 beyond the end of the proximal convoluted tubule increases in a load-dependent manner.(ABSTRACT TRUNCATED AT 250 WORDS) |
| | |
Authors:
|
D C Batlle; Y L Chan |
Related Documents
:
|
1243048 - Potentiated chemotherapeutic activity of 3,6-diamino-10-methyl-acridinium by combinatio... 9925598 - Influence of carbohydrate starvation and arginine on culturability and amino acid utili... 11543338 - Transport of extraterrestrial biomolecules to the earth: problem of thermal stability. 17802428 - Concerted antibiotic effect of penicillin, methionine, threonine and methionine sulfoxi... 2166588 - Inhibitory mechanism of cis-polyunsaturated fatty acids on platelet aggregation: the re... 20149648 - Hydrothermal transformation of chinese privet seed biomass to gas-phase and semi-volati... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Mineral and electrolyte metabolism Volume: 15 ISSN: 0378-0392 ISO Abbreviation: Miner Electrolyte Metab Publication Date: 1989 |
Date Detail:
|
Created Date: 1989-09-08 Completed Date: 1989-09-08 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 7802196 Medline TA: Miner Electrolyte Metab Country: SWITZERLAND |
Other Details:
|
Languages: eng Pagination: 187-94 Citation Subset: IM |
Affiliation:
|
Department of Medicine, Northwestern University Medical School, Chicago, Ill. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Absorption Animals Arginine / pharmacology* Bicarbonates / metabolism* Biological Transport / drug effects Kidney Tubules, Proximal / drug effects, metabolism* Perfusion Rats Rats, Inbred Strains |
| Grant Support | |
ID/Acronym/Agency:
|
AM 27691/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Bicarbonates; 74-79-3/Arginine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Spurious hyperphosphatemia in patients with dysglobulinemia.
Next Document: Rapid separation and quantitation of major phospholipids in biological samples by combined high-perf...