Document Detail


Effect of isocaloric low fat diet on prostate cancer xenograft progression in a hormone deprivation model.
MedLine Citation:
PMID:  20172549     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Previous mouse studies suggesting that low fat diets slow prostate cancer growth often used corn oil (omega-6), which enhances prostate cancer growth, as the primary fat. Using a saturated fat based diet we previously found no significant difference in tumor growth between low and high fat fed SCID mice (Taconic Farms, Hudson, New York) xenografted with LAPC-4 cells. Whether similar results would hold in a castration model is unclear. MATERIALS AND METHODS: A total of 80 male SCID mice were fed a Western diet (40% fat and 44% carbohydrate) and injected with LAPC-4 human prostate cancer cells. When tumors were 200 mm(3), the mice were castrated and randomized to an isocaloric Western or a low fat diet (12% fat and 72% carbohydrate). Animals were sacrificed when tumors were 1,000 mm(3). Serum was collected and assayed for prostate specific antigen, insulin, insulin-like growth factor 1 and insulin-like growth factor binding protein 3. Tumors were assayed for total and phosphorylated Akt. RESULTS: Mouse weight was equivalent in the 2 groups. Overall dietary group was not significantly associated with survival (log rank p = 0.32). There were no statistically significant differences in prostate specific antigen (p = 0.53), insulin-like growth factor axis parameters (each p >0.05) or p-Akt-to-t-Akt ratios (p = 0.22) between the groups at sacrifice. CONCLUSIONS: In this xenograft model we found no difference in tumor growth or survival between low fat vs Western fed mice when the fat source was saturated fat. These results conflict with those of other studies in which corn oil was used to show that low fat diets delay prostate cancer growth, suggesting that fat type may be as important as fat amount in the prostate cancer setting.
Authors:
Jessica C Lloyd; Jodi A Antonelli; Tameika E Phillips; Elizabeth M Masko; Jean-Alfred Thomas; Susan H M Poulton; Michael Pollak; Michael Pollack; Stephen J Freedland
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-02-20
Journal Detail:
Title:  The Journal of urology     Volume:  183     ISSN:  1527-3792     ISO Abbreviation:  J. Urol.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-19     Completed Date:  2010-04-29     Revised Date:  2010-07-21    
Medline Journal Info:
Nlm Unique ID:  0376374     Medline TA:  J Urol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1619-24     Citation Subset:  AIM; IM    
Copyright Information:
Copyright (c) 2010 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.
Affiliation:
Department of Surgery, Durham Veterans Affairs Medical Center, Duke University School of Medicine, Durham, North Carolina, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Diet, Fat-Restricted*
Disease Progression
Energy Intake*
Male
Mice
Mice, SCID
Neoplasm Transplantation
Orchiectomy
Prostatic Neoplasms / diet therapy*
Transplantation, Heterologous
Grant Support
ID/Acronym/Agency:
1 TL1 RR024126/RR/NCRR NIH HHS
Comments/Corrections
Erratum In:
J Urol. 2010 Aug;184(2):808
Note: Pollack, Michael [corrected to Pollak, Michael]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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