Document Detail


The Effect of Intracellular Amino Acids on GSH Production by High-cell-density Cultivation of Saccharomyces cerevisiae.
MedLine Citation:
PMID:  22143994     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The present paper studies the effects of precursor amino acids, i.e., L: -glutamic acid (Glu), L: -glycine (Gly), and L: -cysteine (Cys), on the glutathione (GSH) production. The three amino acids were added during the fermentations. The GSH production was analyzed by gas chromatography-mass spectrometry (GC-MS). It was observed that the cell content of Cys reduced continually, Gly maintained a fairly constant concentration, while Glu remained at a high concentration compared with Cys and Gly. The synthesis of GSH was found to significantly increase after 28 h of fermentation upon addition of 6 mmol l(-1) of each of the three amino acids. Under these conditions, the GSH yields reached 2,250 ± 50 mg l(-1) at 34 h from 1,050 ± 50 mg l(-1) at 28 h. The GC-MS analyses on the effect caused by the addition of amino acids indicated that the addition of Glu was not necessary to improve the GSH production by high-cell-density cultivation of Saccharomyces cerevisiae. The addition of Cys or Gly individually enhances the production of GSH.
Authors:
Miaomiao Wang; Jingfeng Sun; Feiyan Xue; Fei Shang; Zheng Wang; Tianwei Tan
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-6
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  -     ISSN:  1559-0291     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.
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