| The Effect of Intracellular Amino Acids on GSH Production by High-cell-density Cultivation of Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 22143994 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The present paper studies the effects of precursor amino acids, i.e., L: -glutamic acid (Glu), L: -glycine (Gly), and L: -cysteine (Cys), on the glutathione (GSH) production. The three amino acids were added during the fermentations. The GSH production was analyzed by gas chromatography-mass spectrometry (GC-MS). It was observed that the cell content of Cys reduced continually, Gly maintained a fairly constant concentration, while Glu remained at a high concentration compared with Cys and Gly. The synthesis of GSH was found to significantly increase after 28 h of fermentation upon addition of 6 mmol l(-1) of each of the three amino acids. Under these conditions, the GSH yields reached 2,250 ± 50 mg l(-1) at 34 h from 1,050 ± 50 mg l(-1) at 28 h. The GC-MS analyses on the effect caused by the addition of amino acids indicated that the addition of Glu was not necessary to improve the GSH production by high-cell-density cultivation of Saccharomyces cerevisiae. The addition of Cys or Gly individually enhances the production of GSH. |
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Authors:
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Miaomiao Wang; Jingfeng Sun; Feiyan Xue; Fei Shang; Zheng Wang; Tianwei Tan |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-6 |
Journal Detail:
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Title: Applied biochemistry and biotechnology Volume: - ISSN: 1559-0291 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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