| Effect of Hypoxia on Endothelial Nitric Oxide Synthase, NO Production, Intracellular Survival Signaling (p-ERK1/2 and p-AKT) and Apoptosis in Human Term Trophoblast. | |
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MedLine Citation:
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PMID: 20618177 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Citation Park M-H, Galan HL, Arroyo JA. Effect of hypoxia on endothelial nitric oxide synthase, NO production, intracellular survival signaling (p-ERK1/2 and p-AKT) and apoptosis in human term trophoblast. Am J Reprod Immunol 2011; 65: 407-414 Problem Hypoxia is commonly associated with complicated pregnancies such as intrauterine growth restriction. We evaluated the effects of hypoxia on phospho (p)-eNOS, p-ERK, p-AKT and apoptosis in human trophoblast. Method of study Isolated trophoblast were cultured in 21% oxygen or 2% oxygen for 24, 48 and 72 hr. p-eNOS, p-ERK and p-AKT protein were assessed by Western blot and apoptosis by TUNEL assay. NOx was determined in the culture media. Results Compared to controls, hypoxia-exposed CT showed the following: (1) decreased eNOS at 48 and 72 hr, (2) increased p-eNOS at 48 hr, (3) no differences in total NOx production, (4) increased p-ERK at 24, 48 and 72 hr, (5) increased p-AKT at 24 hr (P < 0.05) and (6) increased apoptosis at 48 hr. Conclusion Hypoxia increases activation of p-ERK and induces apoptosis of cultured trophoblast. Hypoxia decreases overall total eNOS but increases p-eNOS, which may allow for NO production to be maintained in trophoblast cells. |
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Authors:
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Mi-Hye Park; Henry L Galan; Juan A Arroyo |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: American journal of reproductive immunology (New York, N.Y. : 1989) Volume: 65 ISSN: 1600-0897 ISO Abbreviation: Am. J. Reprod. Immunol. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-04 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8912860 Medline TA: Am J Reprod Immunol Country: Denmark |
Other Details:
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Languages: eng Pagination: 407-14 Citation Subset: IM |
Copyright Information:
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© 2010 John Wiley & Sons A/S. |
Affiliation:
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From the Division of Perinatal medicine in Departments of Obstetrics and Gynecology, University of Colorado at Denver Health Sciences Center, Denver, CO, USA Department of Obstetrics and Gynecology, Ewha Woman's University, Seoul, Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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