| The effect of human umbilical cord blood cells on survival and cytokine production by post-ischemic astrocytes in vitro. | |
| | |
MedLine Citation:
|
PMID: 20680520 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cerebral ischemia induces death of all neural cell types within the region affected by the loss of blood flow. We have shown that administering human umbilical cord blood cells after a middle cerebral artery occlusion in rats significantly reduces infarct size, presumably by rescuing cells within the penumbra. In this study we examined whether the cord blood cells enhanced astrocyte survival in an in vitro model of hypoxia with reduced glucose availability. Primary astrocyte cultures were incubated for 2 h in no oxygen (95% N, 5% CO(2)) and low glucose (1% compared to 4.5%) media. Cord blood mononuclear cells were added to half the cultures at the beginning of hypoxia. Astrocyte viability was determined using fluorescein diacetate/propidium iodide (FDA/PI) labeling and cytokine production by the astrocytes measured using ELISA. In some studies, T cells, B cells or monocytes/macrophages isolated from the cord blood mononuclear fraction with magnetic antibody cell sorting (MACS) were used instead to determine which cellular component of the cord blood mononuclear fraction was responsible for the observed effects. Co-culturing mononuclear cord blood cells with astrocytes during hypoxia stimulated production of IL-6 and IL-10 during hypoxia. The cord blood T cells decreased survival of the astrocytes after hypoxia but had no effect on the examined cytokines. Our data demonstrate that the tested cord blood fractions do not enhance astrocyte survival when delivered individually, suggesting there is either another cellular component that is neuroprotective or an interaction of all the cells is essential for protection. |
| | |
Authors:
|
Lixian Jiang; Samuel Saporta; Ning Chen; Cyndy Davis Sanberg; Paul Sanberg; Alison Willing |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Stem cell reviews Volume: 6 ISSN: 1558-6804 ISO Abbreviation: Stem Cell Rev Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-10-04 Completed Date: 2011-01-20 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101255952 Medline TA: Stem Cell Rev Country: United States |
Other Details:
|
Languages: eng Pagination: 523-31 Citation Subset: IM |
Affiliation:
|
Center for Excellence in Aging and Brain Repair, University of South Florida, 12901 Bruce B. Downs Blvd, Tampa, FL 33612, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Anoxia / metabolism Astrocytes / cytology*, metabolism Brain Ischemia / metabolism* Cell Survival / physiology Cells, Cultured Cytokines / metabolism* Enzyme-Linked Immunosorbent Assay Fetal Blood / cytology* Humans Interferon-gamma / metabolism Interleukin-10 / metabolism Interleukin-1beta / metabolism Interleukin-6 / metabolism Rats |
| Grant Support | |
ID/Acronym/Agency:
|
R01 NS052839/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Cytokines; 0/Interleukin-1beta; 0/Interleukin-6; 130068-27-8/Interleukin-10; 82115-62-6/Interferon-gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Clinical applications of bedside ultrasonography in internal and emergency medicine.
Next Document: Presentation and outcomes of patients aged 30 years and younger with colorectal cancer: a 20-year r...