Document Detail


Effect of hemoperfusion using polymyxin B-immobilized fibers on non-shock rat sepsis model.
MedLine Citation:
PMID:  20655065     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
BACKGROUND: Direct hemoperfusion with a polymyxin B-immobilized column (PMX-DHP) is recognized to be effective for the treatment of septic shock and is widely applied in Japan. However, it is still unknown whether the efficacy is limited to cardiovascular dysfunction. Therefore, the purpose of this study was to examine the effects of PMX-DHP on a non-hypotensive sepsis model.
METHODS: Wistar rats were assigned to either a PMX-DHP group, control group, or sham group (n = 7 in each group). A sepsis model was made by intravenous infusion of live E. coli. (LD50). The change in systemic blood pressure was less than 20% of the initial level in this model. In the PMX-DHP group, an arteriovenous extracorporeal circuit with a PMX column was applied until 3 h after E. coli injection. The same procedure with a dummy column was applied in the control group. Plasma levels of ALT, LDH, BUN, tumor necrosis factor alpha (TNFα), interleukin (IL)-1β, IL-6, and IL-10 were measured. The mesenteric microcirculation was observed at 1 and 3 h after E. coli injection. In another series, survival was calculated up to 18 h (n = 14 in each group).
RESULTS: Organ damage markers were lower in the PMX-DHP group. The levels of pro-inflammatory cytokines were significantly lower in the PMX-DHP group than in the control group. Microcirculation was better maintained in the PMX-DHP group. Survival was significantly better in the PMX-DHP group (93%) compared with that in the control group (57%, P = 0.03).
CONCLUSIONS: PMX-DHP was effective in a non-hypotensive sepsis model.
Authors:
Toshiaki Iba; Kohei Okamoto; Shiori Kawasaki; Etsuro Nakarai; Taku Miyasho
Related Documents :
18458955 - Comparison of intraperitoneal antithrombin iii and heparin in experimental peritonitis.
3384895 - Rat model for human cryptosporidiosis.
6685695 - Infection of the congenitally athymic rat with mycobacterium leprae.
20354515 - The 'when and whereabouts' of injected pathogen effectors.
22156045 - Acupuncture at st36 prevents chronic stress-induced increases in neuropeptide y in rat.
10499495 - Rats with hypothalamic obesity are insensitive to central leptin injections.
Publication Detail:
Type:  Journal Article     Date:  2010-05-22
Journal Detail:
Title:  The Journal of surgical research     Volume:  171     ISSN:  1095-8673     ISO Abbreviation:  J. Surg. Res.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  755-61     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Affiliation:
Department of Emergency and Disaster Medicine, Juntendo University, Hongo, Bunkyo-ku, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The role of matrix metalloproteinases in intestinal epithelial wound healing during normal and infla...
Next Document:  MELAS syndrome associated with both A3243G-tRNALeu mutation and multiple mitochondrial DNA deletions...