| Effect of growth phase feeding strategies on succinate production by metabolically engineered Escherichia coli. | |
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MedLine Citation:
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PMID: 20038712 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Aerobic growth conditions significantly influenced anaerobic succinate production in two-stage fermentation by Escherichia coli AFP111 with knockouts in rpoS, pflAB, ldhA, and ptsG genes. At a low cell growth rate limited by glucose, enzymes involved in the reductive arm of the tricarboxylic acid cycle and the glyoxylate shunt showed elevated activities, providing AFP111 with intracellular redox balance and increased succinic acid yield and productivity. |
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Authors:
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Min Jiang; Shu-Wen Liu; Jiang-Feng Ma; Ke-Quan Chen; Li Yu; Fang-Fang Yue; Bing Xu; Ping Wei |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-28 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 76 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-08 Completed Date: 2010-04-02 Revised Date: 2010-09-27 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 1298-300 Citation Subset: IM |
Affiliation:
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College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Xinmofan Road #5, Nanjing 210009, China. bioengine@njut.edu.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aerobiosis Anaerobiosis Bacterial Proteins / genetics Citric Acid Cycle Escherichia coli / genetics, growth & development*, metabolism* Fermentation Gene Deletion Genes, Bacterial Genetic Engineering Glucose / metabolism Glyoxylates / metabolism Mutation Oxidation-Reduction Phosphoenolpyruvate Sugar Phosphotransferase System / genetics Sigma Factor / genetics Succinic Acid / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Glyoxylates; 0/Sigma Factor; 0/sigma factor KatF protein, Bacteria; 110-15-6/Succinic Acid; 50-99-7/Glucose; EC 2.7.1.-/Phosphoenolpyruvate Sugar Phosphotransferase System; EC 2.7.1.-/phosphoenolpyruvate-glucose phosphotransferase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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