Document Detail

Effect of GnRH down-regulation on cumulus cell viability and apoptosis as measured by fluorescence-activated cell sorting.
MedLine Citation:
PMID:  18937065     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To determine whether gonadotropin releasing hormone (GnRH)-agonist or -antagonist induces higher percentages of cumulus cell apoptosis and if the use of either is detrimental to ART outcomes.
PATIENTS: Women in a private facility under treatment for IVF had their cumulus cells isolated and analyzed by flow cytometry. Viable, apoptotic, and dead cumulus cell rates related to ovarian stimulation by GnRH-agonist or -antagonist were measured and compared with fertilization and implantation rates.
RESULTS: Treatment with GnRH-agonist produced a greater number of follicles than treatment with GnRH-antagonist. No differences in implantation and pregnancy rates were found. While cumulus cell (CC) apoptosis was positively correlated with estradiol on the day of hCG administration, no significant difference in the percentage of apoptotic cells between treatments was detectable. Additionally, implantation rate and the average follicular estradiol production on the day of hCG administration were no different between treatments.
CONCLUSIONS: GnRH-agonist or -antagonist treatment protocols induce similar levels of apoptosis in CCs and are not detrimental to ART outcomes.
Pericles Assad Hassun Filho; Mariana Antunes Ribeiro; André Monteiro da Rocha; José Roberto Allegreti; Paulo Cesar Serafini; Eduardo Leme Alves da Motta
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Publication Detail:
Type:  Journal Article     Date:  2008-10-21
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  25     ISSN:  1058-0468     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:    2008 Sep-Oct
Date Detail:
Created Date:  2008-11-05     Completed Date:  2009-06-18     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  United States    
Other Details:
Languages:  eng     Pagination:  467-71     Citation Subset:  IM    
Genesis Genetics Brazil, Rua Mato-Grosso, 306, CJ.506 CEP., 01239-040 São Paulo, São Paulo, Brazil.
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MeSH Terms
Apoptosis / drug effects,  physiology*
Cell Survival / drug effects,  physiology*
Cumulus Cells / drug effects,  physiology*
Embryo Implantation
Fertilization in Vitro
Flow Cytometry
Gonadotropin-Releasing Hormone / agonists,  antagonists & inhibitors,  biosynthesis*
Ovulation Induction
Reg. No./Substance:
33515-09-2/Gonadotropin-Releasing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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