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The Effect of Glucosamine Sulfate on the Proliferative Potential and Glycosaminoglycan Synthesis of Nucleus Pulposus Intervertebral Disc Cells.
MedLine Citation:
PMID:  22825479     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
ABSTRACT: Study Design. Based on the similarities in the structure of cartilage and intervertebral disc and on the property of glucosamine of being the building block for the construction of proteoglycan aggregates, we investigated the compound's role in the proliferation of nucleus pulposus cells under iso- and hyperosmotic conditions, the putative activation of signaling cascades and the induction of glycosaminoglycan production.Objective. We aimed to shed light in the mode of action of glucosamine in nucleus pulposus cells.Summary of Background Data. Glucosamine that naturally occurs in cartilage tissues has been widely used for treating osteoarthritis, but its role in nucleus pulposus cells is vastly unknown.Methods. The effect of glucosamine sulfate on the viability and proliferation of nucleus pulposus cells was assessed by the MTT assay, direct cell counting and tritiated thymidine incorporation. Changes in the expression and phosphorylation profile of selected proteins were estimated by western analysis. Glycosaminoglycan production was measured using the Blyscan assay.Results. We showed that glucosamine sulfate up to 1 mM did not influence the viability, proliferation rate or novel DNA synthesis of nucleus pulposus cells in the presence or not of elevated osmolality, but induced the transient phosphorylation of p38 MAPK. The highest concentration used (10 mM) negatively affected cellular proliferation and resulted in de-activation of ERKs and JNKs. Interestingly, these effects turned out to be the outcome of an additional hyperosmotic stress provoked by glucosamine alone. Finally, we found that a long-term incubation with glucosamine leads to an increase in the glycosaminoglycan content of nucleus pulposus cells.Conclusion. Glucosamine sulfate was not found to reverse the high osmolality-mediated delay of proliferation in nucleus pulposus cells needed for the maintenance of the tissue's homeostasis. In addition, glycosaminoglycan synthesis stimulated by glucosamine provides a possible promising clinical role for treating disc degenerative disorders.
Authors:
Eleni Mavrogonatou; Dimitris Kletsas
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-20
Journal Detail:
Title:  Spine     Volume:  -     ISSN:  1528-1159     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7610646     Medline TA:  Spine (Phila Pa 1976)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Laboratory of Cell Proliferation and Ageing, Institute of Biology, National Centre for Scientific Research "Demokritos", 153 10 Athens, Greece.
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