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Effect of Gamma Irradiation on Metallothionein Protein Expression in Plantago ovata Forsk.
MedLine Citation:
PMID:  23020665     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract Purpose : The effect of gamma rays on Metallothionein (MT) expression was studied using the medicinal plant Plantago ovata as the test system. Materials and Methods : Western blotting and Matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) have been used for this purpose. Results: Western blot analysis showed significant induction of metallothionein protein following gamma exposure and that induction was highest at 20 Gy gamma dose. At higher gamma doses (100 Gy) MT expression level declined due to degeneration of cells. MALDI-TOF MS analysis indicated the presence of low molecular weight (7-8 kD) MT molecules following the lower radiation doses. Conclusion : It was concluded from MALDI-TOF MS result that, low gamma exposure leads to expression of MT-like protein. At high doses of gamma ray, MT homologues or MT-like protein was not identified possibly because they might have precipitated due to uncontrolled cross-linking and non-specific aggregation.
Authors:
Priyanka Saha; Debasis Das; Sumana Roy; Arunabha Chakrabarti; Sarmistha Sen Raychaudhuri
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-1
Journal Detail:
Title:  International journal of radiation biology     Volume:  -     ISSN:  1362-3095     ISO Abbreviation:  Int. J. Radiat. Biol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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