| Effect of G-rich oligonucleotides on the proliferation of leukemia cells and its relationship with p53 expression. | |
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MedLine Citation:
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PMID: 21247336 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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G-rich oligonucleotides (GROs) can inhibit cell proliferation by inducing cell cycle arrest at S phase in tumor cell lines. GROs bind specific cellular proteins, such as nucleolin, a crucial protein interacting with P53; however, little is known about the relationship between GROs and P53. In this study, we have shown that GROs inhibited the proliferation of U937 cells (a human monocytic leukemia cell line without P53 expression) by inducing S-phase arrest. We also showed that GRO colocalized with nucleolin in U937 cells. GRO treatment induced alteration of a series of cell cycle regulatory proteins in U937 cells. Increased Cdk2 expression might promote the cells to enter S phase and subsequent decrease of Cdk2 might induce cell cycle arrest in S phase. Transfection of U937 cells with a wild-type p53 gene caused the formation of nucleolin-P53 complex, which alleviated the effect of GRO on leukemia cells. This alleviated effect is probably due to the decreased uptake of GRO. |
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Authors:
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Lei Zhi; Jianwei Zhang; Yujiao Jia; Shilong Shan; Yan Li; Donghai Wang; Min Wang; Qing Rao; Haiyan Xing; Kejing Tang; Zheng Tian; Jianxiang Wang; Yingchang Mi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-01-19 |
Journal Detail:
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Title: Oligonucleotides Volume: 21 ISSN: 1557-8526 ISO Abbreviation: Oligonucleotides Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-02-18 Completed Date: 2011-06-29 Revised Date: 2012-06-25 |
Medline Journal Info:
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Nlm Unique ID: 101188415 Medline TA: Oligonucleotides Country: United States |
Other Details:
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Languages: eng Pagination: 21-7 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biological Transport
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genetics Cell Division / drug effects Cell Proliferation / drug effects Cyclin-Dependent Kinase 2 / genetics, metabolism Cyclins / genetics, metabolism Gene Expression / drug effects Genes, p53 Humans Leukemia, Myeloid / genetics, metabolism, pathology Oligonucleotides / chemical synthesis, genetics, pharmacology* Phosphoproteins / genetics, metabolism* RNA-Binding Proteins / genetics, metabolism* S Phase / drug effects* Transfection Tumor Suppressor Protein p53 / genetics, metabolism* U937 Cells |
| Chemical | |
Reg. No./Substance:
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0/Cyclins; 0/Oligonucleotides; 0/Phosphoproteins; 0/RNA-Binding Proteins; 0/Tumor Suppressor Protein p53; 0/nucleolin; EC 2.7.11.22/CDK2 protein, human; EC 2.7.11.22/Cyclin-Dependent Kinase 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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